肌肌酸激酶(CKM)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Creatine Kinase, Muscle (CKM)
CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed
- 编号KSA109Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围1.56-100ng/mL
- 可适灵敏度0.62ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对肌肌酸激酶(CKM)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
NPA109Ra01 | 肌肌酸激酶(CKM)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA109Ra01 | 肌肌酸激酶(CKM)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA109Ra02 | 肌肌酸激酶(CKM)多克隆抗体 | WB; IHC; ICC; IP. |
PAA109Ra01 | 肌肌酸激酶(CKM)多克隆抗体 | WB; IHC; ICC; IP. |
LAA109Ra71 | 肌肌酸激酶(CKM)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA109Ra21 | 肌肌酸激酶(CKM)单克隆抗体 | WB; IHC; ICC; IP. |
LAA109Ra72 | 肌肌酸激酶(CKM)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA109Ra | 肌肌酸激酶(CKM)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA109Ra | 肌肌酸激酶(CKM)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA109Ra | 肌肌酸激酶(CKM)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA109Ra01 | 肌肌酸激酶(CKM)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA109Ra01 | 肌肌酸激酶(CKM)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Journal of Inflammation | Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery [PubMed: 22583529] |
PLoS One | Extraocular Muscle Characteristics Related to Myasthenia Gravis Susceptibility [PubMed: PMC3568149] |
Kidney Int. | Fructokinase activity mediates dehydration-induced renal injury [Pubmed: 24336030] |
Methylxanthines and cardiotoxicity | Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study [230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf] |
Molecular Medicine Reports | Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473] |
Toxicological Sciences | Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of Carfentanil [Pubmed: 30321427] |
Clinical & Translational Oncology | The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cells [Pubmed: 32447643] |
留言咨询