神经元特异性烯醇化酶(NSE)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Enolase, Neuron Specific (NSE)
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
- 编号KSA537Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.625-40ng/mL
- 可适灵敏度0.231ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对神经元特异性烯醇化酶(NSE)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA537Hu02 | 神经元特异性烯醇化酶(NSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA537Hu01 | 神经元特异性烯醇化酶(NSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA537Hu01 | 神经元特异性烯醇化酶(NSE)多克隆抗体 | WB,IHC |
| PAA537Hu02 | 神经元特异性烯醇化酶(NSE)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA537Hu71 | 神经元特异性烯醇化酶(NSE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA537Hu81 | 神经元特异性烯醇化酶(NSE)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA537Hu27 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu29 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu25 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu21 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu22 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu23 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu26 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu28 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA537Hu24 | 神经元特异性烯醇化酶(NSE)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA537Hu | 神经元特异性烯醇化酶(NSE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA537Hu | 神经元特异性烯醇化酶(NSE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA537Hu01 | 神经元特异性烯醇化酶(NSE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGA537Hu01 | 神经元特异性烯醇化酶(NSE)cDNA | PCR、蛋白表达等 |
参考文献
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| Placenta | Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsia [Pubmed: 24144948] |
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| Analytical Methods | A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read out [Rsc: Source] |
| Int J Neurosci. | The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247] |
| Experimental & Molecular Medicine | Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349] |
| Neuroscience Letters | Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats. [Pubmed:25562631] |
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| Am J Trop Med Hyg | Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in Laos [PubMed: 26055741] |
| Neurosci Lett | Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley rats [PubMed: 25562631] |
| Physiol Rep | S100B and NSE serum concentrations after simulated diving in rats [PubMed: 26462746] |
| Int J Mol Sci | The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions [PubMed: 26184156] |
| PLoS One | Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits [PubMed: 26645542] |
| Фармакология, клиническая фармакология | Волгоградский государственный медицинский университет [uploads:dsovet] |
| Analytical Methods | Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction [articlelanding:c5ay03174e] |
| 中国临床药理学与治疗学 | 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 [article:9794] |
| Asian Pacific Journal of Tropical Medicine | Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury [science:S1995764516300955] |
| Izvorni znanstveni članak | SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI [262238] |
| Neurourol Urodyn | Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid [pubmed:27490041] |
| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830] |
| cellular physiology and biochemistry | Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … [pubmed:29040978] |
| Brain Research | Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway [Pubmed:29510144] |
| Biomed Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336] |
| Journal of the Neurological Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … [Pubmed:29801873] |
| Iranian Journal of Veterinary Research | Nitrosative stress indices in dogs with neurological form of canine distemper [Doi: 10.22099/IJVR.2018.4943] |
| Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway [] |
| The Eurasian Journal of Medicine | Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema [] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the … [] |
| ACTA NEUROBIOLOGIAE EXPERIMENTALIS | Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … [Pubmed: 32214270] |
| Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … [Pubmed: 32300366] |
| European Review for Medical and Pharmacological Sciences | Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … [Pubmed: 32432773] |
| Neurobiology of Learning and Memory | Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats [] |
| The role of light desynchronosis in the development of stress-induced aging [] | |
| ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ … [] | |
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| J Surg Res | Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway [34399353] |
| Cephalalgia | Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome [34644195] |
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