免疫球蛋白E(IgE)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Immunoglobulin E (IgE)
IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region
- 编号KSA545Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围1.56-100ng/mL
- 可适灵敏度0.69ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对免疫球蛋白E(IgE)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
NPA545Hu01 | 免疫球蛋白E(IgE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA545Hu01 | 免疫球蛋白E(IgE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA545Hu01 | 免疫球蛋白E(IgE)多克隆抗体 | WB; IHC; ICC; IP. |
LAA545Hu71 | 免疫球蛋白E(IgE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
RAA545Hu21 | 免疫球蛋白E(IgE)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
MAA545Hu22 | 免疫球蛋白E(IgE)单克隆抗体 | WB; IHC; ICC; IP. |
SEA545Hu | 免疫球蛋白E(IgE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA545Hu | 免疫球蛋白E(IgE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA545Hu01 | 免疫球蛋白E(IgE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Molecular Carcinogenesis | IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection [PubMed: 23280722] |
Human Immunology | IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection [PubMed: 23354321] |
Scand J Immunol | Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation [Pubmed: 24313384] |
AAPS PharmSciTech | Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema [PubMed: 25511806] |
Ann Allergy Asthma Immunol | Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation [PubMed: 26123423] |
Molecular Medicine Reports | The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. [pubmed:27484042] |
Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
International Immunopharmacology | Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung [pubmed:27992791] |
Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
Molecular Medicine Reports | Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. [pubmed:28586019] |
Journal of Autoimmunity | Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003] |
Water Research | Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095] |
Human & Experimental Toxicology | Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles [Pubmed:29233007] |
Journal of International Medical Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127] |
Journal Of Cellular Biochemistry | Effects of catalpol on bronchial asthma and its relationship with cytokines [Pubmed: 30536454] |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
Royal Society Open Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
Health and Medicine | Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis [] |
American Journal of Translational Research | Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193] |
MOLECULAR IMMUNOLOGY | Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806] |
bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
international journal of antimicrobial agents | Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo [Pubmed: 32335278] |
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
Biomarkers of Allergic Asthma and their Association with Serum Parameters [] | |
Biomed Pharmacother | Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice [33706133] |
Environ Pollut | Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073] |
Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity [] |
Mediators Inflamm | Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps [Pubmed:35075349] |
Expert Opinion on Therapeutic Targets | Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma [Pubmed:35549595] |
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