异柠檬酸脱氢酶1(IDH1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Isocitrate Dehydrogenase 1, Soluble (IDH1)
PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase
- 编号KSH839Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.781-50ng/mL
- 可适灵敏度0.37ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7560 ¥ 12600 ¥ 22680 ¥ 44100 ¥ 75600
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特异性
试剂盒中的抗体对异柠檬酸脱氢酶1(IDH1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPH839Hu01 | 异柠檬酸脱氢酶1(IDH1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAH839Hu01 | 异柠檬酸脱氢酶1(IDH1)多克隆抗体 | WB,IHC |
MAH839Hu22 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC |
MAH839Hu23 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC; ICC; IP. |
MAH839Hu25 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC; ICC; IP. |
MAH839Hu27 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC; ICC; IP. |
MAH839Hu29 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC; ICC; IP. |
MAH839Hu24 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC |
MAH839Hu26 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC |
MAH839Hu21 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体 | WB; IHC; ICC; IP. |
RAH839Hu21 | 异柠檬酸脱氢酶1(IDH1)重组抗体 | WB; IFC; IHC; IP. |
LAH839Hu76 | 异柠檬酸脱氢酶1(IDH1)单克隆抗体(生物素标记) | WB; IHC; ICC. |
IEH839Hu | 异柠檬酸脱氢酶1(IDH1)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEH839Hu | 异柠檬酸脱氢酶1(IDH1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCH839Hu | 异柠檬酸脱氢酶1(IDH1)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMH839Hu | 异柠檬酸脱氢酶1(IDH1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSH839Hu01 | 异柠檬酸脱氢酶1(IDH1)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSH839Hu01 | 异柠檬酸脱氢酶1(IDH1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Molecular & Cellular Proteomics | Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic Analysis [Mcponline: 008821] |
Molecular & Cellular Proteomics | Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic Analysis [Pubmed: 22064513] |
Clinical Cancer Research | Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancer [Pubmed: 24046070] |
Onco Targets Ther | The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells [PubMed: 25914546] |
Oncogene | Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanism [Pubmed:29921847] |
Molecular Oncology | IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer [Pubmed:29465809] |
Cancers | Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver … [Pubmed: 32443439] |
International Journal of Cancer | Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer [Pubmed:35689436] |
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