基质Gla蛋白(MGP)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Matrix Gla Protein (MGP)
MGLAP; GIG36; Cell growth-inhibiting gene 36 protein
- 编号KSB477Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.625-40ng/mL
- 可适灵敏度0.254ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对基质Gla蛋白(MGP)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB477Hu01 | 基质Gla蛋白(MGP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB477Hu01 | 基质Gla蛋白(MGP)多克隆抗体 | WB; IHC; ICC; IP. |
MAB477Hu22 | 基质Gla蛋白(MGP)单克隆抗体 | WB; IHC; ICC; IP. |
SEB477Hu | 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB477Hu | 基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSB477Hu01 | 基质Gla蛋白(MGP)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB477Hu01 | 基质Gla蛋白(MGP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Nephrology Dialysis Transplantation | Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells [PubMed: PMC3611891] |
Maturitas. | Bone metabolism regulators and arterial stiffness in postmenopausal women [Pubmed: 23916080] |
The Egyptian Rheumatologist | Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity [ScienceDirect: S1110116414000076] |
Toxicology Reports | Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice [Researchgate:Source] |
Lipids in Health and Disease | Markers of increased cardiovascular risk in patients with chronic kidney disease [Biomedcentral:Source] |
Human & Veterinary Medicine | The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins [Hvm:Source] |
Pol Arch Med Wewn | Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5 [PubMed: 26176325] |
Lipids Health Dis. | Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study [pmc:PMC4739105] |
Kidney Blood Press Res. | Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease [Pubmed:27100101] |
Archives of Oral Biology | Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus [pubmed:27348051] |
Cell Death & Disease | Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis [Pubmed:29880866] |
Urology | High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein [Pubmed:29935264] |
Clinica Chimica Acta | Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases [Pubmed: 30597137] |
Biological Trace Element Research | Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765] |
Renal Failure | Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients [Pubmed: 31538831] |
Heliyon | Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 [Pubmed: 32322728] |
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