粘蛋白5AC(MUC5AC)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- 编号KSA756Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.064ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6998 ¥ 11664 ¥ 20995 ¥ 40824 ¥ 69984
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对粘蛋白5AC(MUC5AC)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA756Mu02 | 粘蛋白5AC(MUC5AC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA756Mu01 | 粘蛋白5AC(MUC5AC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA756Mu03 | 粘蛋白5AC(MUC5AC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA756Mu01 | 粘蛋白5AC(MUC5AC)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA756Mu02 | 粘蛋白5AC(MUC5AC)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA756Mu03 | 粘蛋白5AC(MUC5AC)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA756Mu81 | 粘蛋白5AC(MUC5AC)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA756Mu71 | 粘蛋白5AC(MUC5AC)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| IEA756Mu | 粘蛋白5AC(MUC5AC)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA756Mu | 粘蛋白5AC(MUC5AC)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA756Mu | 粘蛋白5AC(MUC5AC)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA756Mu | 粘蛋白5AC(MUC5AC)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA756Mu01 | 粘蛋白5AC(MUC5AC)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| Mucosal Immunology | Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13 [Nature: 201082a] |
| European Journal of Pharmacology | Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways [ScienceDirect: S0014299912005523] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
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| Deletion Polymorphism and Chronic Obstructive Pulmonary Disease | ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells [Pubmed:27075970] |
| Int J Clin Exp Med | The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai [files:ijcem0020163.pdf] |
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| J Virol. | Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. [pubmed:28275196] |
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| Journal of Immunology Research | Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse models [pubmed:28835901] |
| BASIC INVESTIGATION | Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases [pubmed:28644234] |
| International Immunopharmacology | Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway [pubmed:28550735] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158] | |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Respiratory Research | IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells [pubmed:28931396] |
| Chemotherapy | Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. [pubmed:28578323] |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623] |
| European Journal of Pharmacology | Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling [Pubmed:29382534] |
| Inflammation | Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in … [Pubmed:29349683] |
| Pulmonary Pharmacology and Therapeutics | In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts [Pubmed:29698781] |
| European Respiratory Society | Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion [Pubmed:29946002] |
| Clinical and Experimental Ophthalmology | Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer strips [Pubmed: 30214141] |
| international journal of biological macromolecules | Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancer [Pubmed: 30287374] |
| Journal of Investigative Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| Phytomedicine | Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretion [Pubmed: 31301536] |
| Scientific Reports | Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5AC [Pubmed: 32076085] |
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| Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice [] | |
| Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡ [33285209] |
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| Cell Rep | Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs [33535050] |
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