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神经生长因子(NGF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

  • 神经生长因子(NGF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 神经生长因子(NGF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对神经生长因子(NGF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA105Ra01 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA105Ra01 神经生长因子(NGF)活性蛋白 Cell culture; Activity Assays.
EPA105Ra61 神经生长因子(NGF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA105Ra01 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
MAA105Ra22 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
MAA105Ra21 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
SEA105Ra 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA105Ra 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA105Ra 神经生长因子(NGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA105Ra 神经生长因子(NGF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA105Ra01 神经生长因子(NGF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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International Journal of Pharmaceutics Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs [Pubmed:25445537]
Yonsei Medical Journal NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766]
Molecular and Cellular Endocrinology High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971]
Mol Cell Endocrinol High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971]
Neurosciences (Riyadh) Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. [PubMed: 25864063]
Neurotoxicity Research Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures [Pubmed:27189478]
Psychoneuroendocrinology Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism [Pubmed:26771945]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities [pubmed:28081639]
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Osteoarthritis and Cartilage Mechanical loading of intervertebral disc modulates microglia proliferation, activation, and chemotaxis [Pubmed:29723636]
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Experimental Dermatology 3‐‐Laurylglyceryl ascorbate improves the development of sensitive skin through the reduction of oxidative stress [Pubmed: 30698879]
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Experimental and Therapeutic Medicine Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity [Pubmed: 32010279]
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MOLECULAR NUTRITION & FOOD RESEARCH Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … [Pubmed: 32918393]
Neuropharmacology Impaired LTD-like motor cortical plasticity in female patients with major depression disorder [Pubmed: 32791084]
COLLOIDS AND SURFACES B-BIOINTERFACES Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease [Pubmed: 32777662]
博士论文 Neurobiological determinants of depressive-like symptoms in rodents []
Medical Research Journal Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy []
Journal of the Taiwan Institute of Chemical Engineers Multiple-component dual-phase solid lipid nanoparticles with conjugated transferrin for formulating antioxidants and nerve growth factor against neuronal apoptosis []
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­ [33389158]
DRUG DEVELOPMENT RESEARCH Neuroprotective effect of Na+/H+?exchangers isoform©\1 inactivation against 6©\hydroxydopamine©\induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models [33538000]
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways [33589896]
Neuropsychiatr Dis Treat Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 … [34239303]
Pharmaceuticals (Basel) Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers [34959705]
Cell Biochem Funct CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452]
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