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骨粘连蛋白(ON)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Osteonectin (ON)

SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine

  • 骨粘连蛋白(ON)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨粘连蛋白(ON)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对骨粘连蛋白(ON)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
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LMA791Mu 骨粘连蛋白(ON)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
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参考文献

杂志 参考文献
J Mol Med (Berl) Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance [PubMed: 26199111]
Muscle Nerve Effect of resistance ladder training on SPARC expression in skeletal muscle of hindlimb immobilized rats [PubMed: 26467437]
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
British Journal of Cancer A proteomics-based approach identifies secreted protein acidic and rich in cysteine as a prognostic biomarker in malignant pleural mesothelioma [Pubmed:26889976]
Journal of diabetes and its complications Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. [pubmed:27916484]
American journal of the medical sciences Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus [pubmed:29078839]
Translational Research in Oral Oncology Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer [Doi: 10.1177/2057178X18800534]
[ENGINEERING OF BIOMATERIALS Osteoconductive potential of PLGA/bioglass composite biomaterials []
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