血小板因子4(PF4)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Platelet Factor 4 (PF4)
CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
- 编号KSA172Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, platelet-poor plasma and cell culture supernates.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围1.56-100pg/mL
- 可适灵敏度0.65pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
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特异性
试剂盒中的抗体对血小板因子4(PF4)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
APA172Hu01 | 血小板因子4(PF4)活性蛋白 | Cell culture; Activity Assays. |
RPA172Hu01 | 血小板因子4(PF4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA172Hu02 | 血小板因子4(PF4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA172Hu01 | 血小板因子4(PF4)多克隆抗体 | WB; IHC; ICC; IP. |
LAA172Hu71 | 血小板因子4(PF4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA172Hu81 | 血小板因子4(PF4)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAA172Hu21 | 血小板因子4(PF4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA172Hu23 | 血小板因子4(PF4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA172Hu24 | 血小板因子4(PF4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA172Hu22 | 血小板因子4(PF4)单克隆抗体 | WB; IHC |
MAA172Hu25 | 血小板因子4(PF4)单克隆抗体 | WB; IHC; ICC; IP. |
SEA172Hu | 血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA172Hu | 抗血小板因子4抗体(Anti-PF4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
SCA172Hu | 血小板因子4(PF4)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA172Hu | 血小板因子4(PF4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA172Hu01 | 血小板因子4(PF4)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA172Hu01 | 血小板因子4(PF4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics | Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats [PubMed: 20601223] |
Basic Research in Cardiology | Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170] |
PLoS One | Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation [PubMed: PMC3608633] |
Diving and Hyperbaric Medicine | Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression [Pubmed:25311322] |
Journal of Functional Foods | A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivo [science:S1756464616300676] |
Plos one | Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury [pubmed:27583400] |
international journal of molecular sciences | Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. [pubmed:28272338] |
Experimental and Therapeutic Medicine | Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability [pubmed:28450960] |
Reproductive Toxicology | Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats [Pubmed: 30528429] |
INTERNATIONAL JOURNAL OF CARDIOLOGY | Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation [Pubmed: 30857843] |
Available at SSRN 3335365 | Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers [] |
journal of biotechnology | Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis [Pubmed: 30927490] |
Data in Brief | Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation [] |
journal of thoracic and cardiovascular surgery | Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers [Pubmed: 32812629] |
Marine Drugs | The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230] |
Thrombosis Research | Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449] |
Nat Commun | The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets [34099640] |
Vaccine | The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388] |
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