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视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Retinol Binding Protein 4 (RBP4)

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

  • 视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对视黄醇结合蛋白4(RBP4)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Bos taurus; Bovine (Cattle，牛) 应用(仅供研究使用，不用于临床诊断！)
RPA929Bo01 视黄醇结合蛋白4(RBP4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA929Bo02 视黄醇结合蛋白4(RBP4)活性蛋白 Cell culture; Activity Assays.
PAA929Bo01 视黄醇结合蛋白4(RBP4)多克隆抗体 WB; IHC
PAA929Bo02 视黄醇结合蛋白4(RBP4)多克隆抗体 WB; IHC; ICC; IP.
LAA929Bo81 视黄醇结合蛋白4(RBP4)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA929Bo71 视黄醇结合蛋白4(RBP4)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA929Bo21 视黄醇结合蛋白4(RBP4)单克隆抗体 WB; IHC; ICC; IP.
SEA929Bo 视黄醇结合蛋白4(RBP4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA929Bo 视黄醇结合蛋白4(RBP4)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA929Bo01 视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Diabetes Technology & Therapeutics Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078]
Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 [PubMed: 21130593]
Evid Based Complement Alternat Med Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats [PubMed: PMC3368504]
Journal of Endocrinology Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles [Pubmed:24501381]
J Endocrinol Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061]
Peptides Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats [Pubmed:25445608]
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173]
Experimental and Therapeutic Medicine Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone [Pubmed:27446311]
Experimental and Therapeutic Medicine Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. [pubmed:27446311]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
BMC Cancer Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis [Pubmed:29386009]
Nutrition & Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416]
Molecular Medicine Reports RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway [Pubmed:30015949]
Relationship between retinol and risk of diabetic retinopathy: a case-control study []
Scientific Reports Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle [Pubmed: 31147589]
Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals []
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma []
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861]
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274]
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309]
Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507]
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