视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Retinol Binding Protein 4 (RBP4)
PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma
- 编号KSA929Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.625-40ng/mL
- 可适灵敏度0.292ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 5378 ¥ 8964 ¥ 16135 ¥ 31374 ¥ 53784
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对视黄醇结合蛋白4(RBP4)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA929Hu01 | 视黄醇结合蛋白4(RBP4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA929Hu61 | 视黄醇结合蛋白4(RBP4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA929Hu01 | 视黄醇结合蛋白4(RBP4)活性蛋白 | Cell culture; Activity Assays. |
APA929Hu61 | 视黄醇结合蛋白4(RBP4)活性蛋白 | Cell culture; Activity Assays. |
PAA929Hu06 | 视黄醇结合蛋白4(RBP4)多克隆抗体 | WB; IHC; ICC; IP. |
PAA929Hu01 | 视黄醇结合蛋白4(RBP4)多克隆抗体 | WB; IHC; ICC; IP. |
LAA929Hu81 | 视黄醇结合蛋白4(RBP4)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA929Hu71 | 视黄醇结合蛋白4(RBP4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA929Hu22 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB,IHC |
MAA929Hu26 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA929Hu21 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA929Hu23 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA929Hu29 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA929Hu25 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB |
MAA929Hu27 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB; IHC; ICC; IP. |
MAA929Hu24 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB; IHC; ICC; IP. |
FAA929Hu02 | 抗视黄醇结合蛋白4(RBP4)单克隆抗体 | FCM |
SEA929Hu | 视黄醇结合蛋白4(RBP4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA929Hu | 视黄醇结合蛋白4(RBP4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA929Hu01 | 视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Diabetes Technology & Therapeutics | Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078] |
Journal of Pharmaceutical and Biomedical Analysis | Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 [PubMed: 21130593] |
Evid Based Complement Alternat Med | Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats [PubMed: PMC3368504] |
Journal of Endocrinology | Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles [Pubmed:24501381] |
J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061] |
Peptides | Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats [Pubmed:25445608] |
Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126] |
Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173] |
Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone [Pubmed:27446311] |
Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. [pubmed:27446311] |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis [Pubmed:29386009] |
Nutrition & Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416] |
Molecular Medicine Reports | RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway [Pubmed:30015949] |
Relationship between retinol and risk of diabetic retinopathy: a case-control study [] | |
Scientific Reports | Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle [Pubmed: 31147589] |
Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals [] | |
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma [] | |
Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861] |
Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274] |
Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507] |
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