泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
- 编号KSG945Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.78-50ng/mL
- 可适灵敏度0.32ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7776 ¥ 12960 ¥ 23328 ¥ 45360 ¥ 77760
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对泛素羧基端酯酶L1(UCHL1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPG945Mu02 | 泛素羧基端酯酶L1(UCHL1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPG945Mu01 | 泛素羧基端酯酶L1(UCHL1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAG945Mu01 | 泛素羧基端酯酶L1(UCHL1)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAG945Mu02 | 泛素羧基端酯酶L1(UCHL1)多克隆抗体 | WB; IHC; ICC; IP. |
| SEG945Mu | 泛素羧基端酯酶L1(UCHL1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMG945Mu | 泛素羧基端酯酶L1(UCHL1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSG945Mu01 | 泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084] |
| Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533] |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135] |
| Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290] |
| Intensive Care Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900] |
| Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088] |
| Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9] | |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448] |
| Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792] |
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