酸性磷酸酶1(ACP1)活性蛋白
Active Acid Phosphatase 1 (ACP1)
HAAP; Low Molecular Weight Phosphotyrosine Protein Phosphatase; Adipocyte acid phosphatase; Red cell acid phosphatase 1; LMW-PTPase
- 编号APA994Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点6.7
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1008 ¥ 2520 ¥ 5040 ¥ 15120 ¥ 37800
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure. SDS-PAGE
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Acid phosphatase locus 1 (ACP1) is a low molecular weight protein tyrosine phosphatase that has been shown to be an important regulator of insulin receptor signaling. EFNA1 is a kind of receptor tyrosine kinase which can Interact with ACP1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA1 and recombinant human ACP1. Briefly, EFNA1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACP1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-EFNA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. When recombinant human ACP1 is immobilized at 2 ug/ml (100 ul/well), the concentration of EFNA1 that produces 50% optimal binding response is found to be approximately 0.21 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA994Hu02 | 酸性磷酸酶1(ACP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA994Hu01 | 酸性磷酸酶1(ACP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA994Hu01 | 酸性磷酸酶1(ACP1)活性蛋白 | Cell culture; Activity Assays. |
| PAA994Hu02 | 酸性磷酸酶1(ACP1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA994Hu01 | 酸性磷酸酶1(ACP1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA994Hu81 | 酸性磷酸酶1(ACP1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA994Hu71 | 酸性磷酸酶1(ACP1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA994Hu21 | 酸性磷酸酶1(ACP1)单克隆抗体 | WB |
| MAA994Hu22 | 酸性磷酸酶1(ACP1)单克隆抗体 | WB; IHC; ICC; IP. |
| KSA994Hu01 | 酸性磷酸酶1(ACP1)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |