太阳集团tyc5997_平台(china)官方网站

抗凝血酶(AT)活性蛋白

Active Antithrombin (AT)

AT3; ATIII; SERPINC1; Anti-Thrombin Antibodies; Serpin Peptidase Inhibitor Clade C Member 1; Coding Sequence Signal Peptide Antithrombin Part 1

  • 抗凝血酶(AT)活性蛋白 产品包装(模拟)
  • 抗凝血酶(AT)活性蛋白 产品包装(模拟)
  • APC313Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Antithrombin III, also known as Serpin C1, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases. For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery. The activity of recombinant human antithrombin III was measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Thrombin was diluted to 0.5 U with heparin at 50 µg/ml in the assay buffer and 10 ul different concentrations of recombinant human antithrombin III (MW: 50 KD) was incubated with 10 ul diluted thrombin at 37 ℃ for 30 minutes. Loading 50 µL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 µL of 200 µM substrate. Include a substrate blank containing 50 µL of assay buffer and 50 µL of 200 µM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human antithrombin III significantly decreased thrombin activity. The inhibition IC50 was <8 nM.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
NPC313Hu01 抗凝血酶(AT)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC313Hu01 抗凝血酶(AT)活性蛋白 Cell culture; Activity Assays.
RPC313Hu03 抗凝血酶(AT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPC313Hu61 抗凝血酶(AT)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC313Hu01 抗凝血酶(AT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC313Hu02 抗凝血酶(AT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC313Hu61 抗凝血酶(AT)活性蛋白 Cell culture; Activity Assays.
PAC313Hu01 抗凝血酶(AT)多克隆抗体 WB; IHC; ICC; IP.
PAC313Hu02 抗凝血酶(AT)多克隆抗体 WB; IHC; ICC; IP.
PAC313Hu03 抗凝血酶(AT)多克隆抗体 WB; IHC; ICC; IP.
LAC313Hu81 抗凝血酶(AT)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAC313Hu71 抗凝血酶(AT)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC313Hu21 抗凝血酶(AT)单克隆抗体 WB; IHC; ICC; IP.
MAC313Hu27 抗凝血酶(AT)单克隆抗体 WB,IHC
MAC313Hu29 抗凝血酶(AT)单克隆抗体 WB; IHC; ICC; IP.
MAC313Hu23 抗凝血酶(AT)单克隆抗体 WB; IHC; ICC; IP.
MAC313Hu24 抗凝血酶(AT)单克隆抗体 WB; IHC; ICC; IP.
MAC313Hu26 抗凝血酶(AT)单克隆抗体 WB; IHC; ICC; IP.
MAC313Hu22 抗凝血酶(AT)单克隆抗体 WB,IHC
MAC313Hu28 抗凝血酶(AT)单克隆抗体 WB; IHC; ICC; IP.
MAC313Hu25 抗凝血酶(AT)单克隆抗体 WB; IHC; ICC; IP.
FAC313Hu02 抗抗凝血酶(AT)单克隆抗体 FCM
FAC313Hu04 抗抗凝血酶(AT)单克隆抗体 FCM
FAC313Hu08 抗抗凝血酶(AT)单克隆抗体 FCM
LAC313Hu72 抗凝血酶(AT)单克隆抗体(生物素标记) WB; IHC; ICC.
LAC313Hu82 抗凝血酶(AT)单克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEC313Hu 抗凝血酶(AT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC313Hu 抗凝血酶(AT)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC313Hu 抗凝血酶(AT)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSC313Hu01 抗凝血酶(AT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Thromb Res Thalidomide and multiple myeloma serum synergistically induce a hemostatic imbalance in endothelial cells in vitro [PubMed: 25840743]
Neurotherapeutics iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management [pubmed:29247448]
Journal of Clinical and Aesthetic Dermatology Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar. []
博士论文 An Agile Approach to Novel Recombinant Antithrombin Production for use in Biologics Development []
留言咨询
Baidu
sogou