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抑肽酶(AP)活性蛋白

Active Aprotinin (AP)

BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor

  • 抑肽酶(AP)活性蛋白 产品包装(模拟)
  • 抑肽酶(AP)活性蛋白 产品包装(模拟)
  • APA968Bo61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant bovine AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 μl 4 mg/mL trypsin diluted by 1mM HCl to 160 μl assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100 µl of 1mg/ml BAPNA. Include a substrate blank containing 160 μl assay buffer, 20 μl 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100 µL of 1mg/ml substrate. Rapidly mixing at 25 ℃, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ∆A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant bovine AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25℃ for 10 minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant bovine AP is >2000 U/mg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
NPA968Bo01 抑肽酶(AP)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA968Bo62 抑肽酶(AP)活性蛋白 Cell culture; Activity Assays.
EPA968Bo62 抑肽酶(AP)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA968Bo61 抑肽酶(AP)活性蛋白 Cell culture; Activity Assays.
EPA968Bo61 抑肽酶(AP)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA968Bo01 抑肽酶(AP)活性蛋白 Cell culture; Activity Assays.
RPA968Bo01 抑肽酶(AP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA968Bo01 抑肽酶(AP)多克隆抗体 WB; IHC; ICC; IP.
LAA968Bo71 抑肽酶(AP)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA968Bo21 抑肽酶(AP)单克隆抗体 WB; IHC; ICC; IP.
CEA968Bo 抑肽酶(AP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA968Bo 抑肽酶(AP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA968Bo 抑肽酶(AP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA968Bo11 抑肽酶(AP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
24 Aprotinin prevents proteolytic epithelial sodium channel (ENaC) activation and volume retention in nephrotic syndrome. [pubmed:29042083]
Aus der Medizinischen Universitätsklinik diss_Menacher word []
Acta Physiologica Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome [Pubmed: 32455507]
Evaluation of The Relationship Between Apelin 36 and Oxidative Stress In Patients With General Anxiety Disorder []
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