太阳集团tyc5997_平台(china)官方网站

精氨酸酶(ARG)活性蛋白

Active Arginase (ARG)

ARG1; Arginase I; Liver Arginase

  • 精氨酸酶(ARG)活性蛋白 产品包装(模拟)
  • 精氨酸酶(ARG)活性蛋白 产品包装(模拟)
  • APB120Ra01.jpg SDS-PAGE图
  • 精氨酸酶(ARG)活性蛋白 Figure. Western Blot; Sample: Recombinant Arg, Rat.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Estrogen Receptor Alpha (ERa) has been identified as an interactor of, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Arginase( Arg) and recombinant rat ERa. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ERa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and ERa was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB120Ra01 精氨酸酶(ARG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB120Ra01 精氨酸酶(ARG)活性蛋白 Cell culture; Activity Assays.
PAB120Ra01 精氨酸酶(ARG)多克隆抗体 WB; IHC; ICC; IP.
LAB120Ra71 精氨酸酶(ARG)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB120Ra81 精氨酸酶(ARG)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB120Ra23 精氨酸酶(ARG)单克隆抗体 WB; IHC; ICC; IP.
MAB120Ra24 精氨酸酶(ARG)单克隆抗体 WB; IHC; ICC; IP.
MAB120Ra22 精氨酸酶(ARG)单克隆抗体 WB; IHC; ICC; IP.
MAB120Ra21 精氨酸酶(ARG)单克隆抗体 WB; IHC; ICC; IP.
SEB120Ra 精氨酸酶(ARG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB120Ra 精氨酸酶(ARG)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB120Ra01 精氨酸酶(ARG)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
PLoS One Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers [Pubmed: 24204595]
J Cereb Blood Flow Metab Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke [PubMed: 25966956]
Cancer Medicine Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease [Pubmed:27109254]
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496]
Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells []
Commun Biol Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells [34893694]
留言咨询
Baidu
sogou