钙蛋白酶1(CAPN1)活性蛋白
Active Calpain 1 (CAPN1)
PIG30; CANPL1; MuCANP; MuCL; Calpain 1, Large Subunit; Calpain-1 Catalytic Subunit; Calcium-Dependent Protease 1; Calcium-activated neutral proteinase 1; Calpain mu-type; Micromolar-calpain
- 编号APB964Ra01
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点5.3
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Gene sequencing
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant CAPN1, Rat.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APB964Ra01 | 钙蛋白酶1(CAPN1)活性蛋白 | Cell culture; Activity Assays. |
| APB964Ra02 | 钙蛋白酶1(CAPN1)活性蛋白 | Cell culture; Activity Assays. |
| RPB964Ra01 | 钙蛋白酶1(CAPN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB964Ra02 | 钙蛋白酶1(CAPN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB964Ra01 | 钙蛋白酶1(CAPN1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB964Ra02 | 钙蛋白酶1(CAPN1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB964Ra71 | 钙蛋白酶1(CAPN1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB964Ra21 | 钙蛋白酶1(CAPN1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB964Ra | 钙蛋白酶1(CAPN1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB964Ra | 钙蛋白酶1(CAPN1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Bulletin du Cancer | Calpain 1 in bronchoalveolar lavage fluid is associated with poor prognosis in lepidic predominant pulmonary adenocarcinoma [Pubmed: 30683309] |