凝血因子Ⅱ(F2)活性蛋白
Active Coagulation Factor II (F2)
FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2
- 编号APA820Ra02
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点4.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2304 ¥ 5760 ¥ 11520 ¥ 34560 ¥ 86400
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产品包装(模拟)
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产品包装(模拟)
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Gene sequencing
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant F2, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Coagulation Factor II (F2) also commonly called pro-thrombin is a coagulation protein in the blood stream that has many effects in the coagulation cascade. It is a serine protease that converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Besides, Protein C Inhibitor (PCI) has been identified as an interactor of F2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat F2 and recombinant rat PCI. Briefly, F2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PCI-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-F2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of F2 and PCI was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA820Ra03 | 凝血因子Ⅱ(F2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA820Ra02 | 凝血因子Ⅱ(F2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA820Ra01 | 凝血因子Ⅱ(F2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA820Ra02 | 凝血因子Ⅱ(F2)活性蛋白 | Cell culture; Activity Assays. |
| PAA820Ra02 | 凝血因子Ⅱ(F2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA820Ra03 | 凝血因子Ⅱ(F2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA820Ra01 | 凝血因子Ⅱ(F2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA820Ra71 | 凝血因子Ⅱ(F2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA820Ra81 | 凝血因子Ⅱ(F2)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA820Ra29 | 凝血因子Ⅱ(F2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA820Ra21 | 凝血因子Ⅱ(F2)单克隆抗体 | WB |
| MAA820Ra23 | 凝血因子Ⅱ(F2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA820Ra24 | 凝血因子Ⅱ(F2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA820Ra25 | 凝血因子Ⅱ(F2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA820Ra27 | 凝血因子Ⅱ(F2)单克隆抗体 | WB; IHC |
| MAA820Ra22 | 凝血因子Ⅱ(F2)单克隆抗体 | WB,IHC |
| SEA820Ra | 凝血因子Ⅱ(F2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA820Ra | 凝血因子Ⅱ(F2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA820Ra01 | 凝血因子Ⅱ(F2)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Blood Cells Mol Dis | Upregulation of cytokine signalling in platelets increases risk of thrombophilia in severe COVID-19 patients [Pubmed:35180460] |