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凝血因子Ⅱ(F2)活性蛋白

Active Coagulation Factor II (F2)

FII; TM; PT; Thrombin; Prothrombin; Pro-Thrombin; Activation peptide fragment 1; Activation peptide fragment 2

  • 凝血因子Ⅱ(F2)活性蛋白 产品包装(模拟)
  • 凝血因子Ⅱ(F2)活性蛋白 产品包装(模拟)
  • 凝血因子Ⅱ(F2)活性蛋白 Gene sequencing
  • APA820Ra02.jpg SDS-PAGE图
  • 凝血因子Ⅱ(F2)活性蛋白 Figure. Western Blot; Sample: Recombinant F2, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Coagulation Factor II (F2) also commonly called pro-thrombin is a coagulation protein in the blood stream that has many effects in the coagulation cascade. It is a serine protease that converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Besides, Protein C Inhibitor (PCI) has been identified as an interactor of F2, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat F2 and recombinant rat PCI. Briefly, F2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PCI-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-F2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of F2 and PCI was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

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参考文献

杂志 参考文献
Blood Cells Mol Dis Upregulation of cytokine signalling in platelets increases risk of thrombophilia in severe COVID-19 patients [Pubmed:35180460]
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