二胺氧化酶(ABP1)活性蛋白
Active Amiloride Binding Protein 1 (ABP1)
AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1
- 编号APA656Hu61
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 5% Trehalose.
- 性状冻干粉
- 纯度> 97%
- 等电点6.6
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1805 ¥ 4512 ¥ 9024 ¥ 27072 ¥ 67680
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产品包装(模拟)
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产品包装(模拟)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Amiloride binding protein (ABP1), also known as amine oxidase copper domain-containing protein 1 (AOC1), is an approximately 110 kDa member of the copper-containing amine oxidase family. ABP1 is a glycosylated enzyme that forms disulfide-linked homodimers. It is stored in cytoplasmic granules of epithelial cells in the kidney, placenta, uterus, lung, and intestine, and its extracellular release can be induced by heparin. ABP1 exhibits a substrate preference for histamine and putrescine, generating hydrogen peroxide and ammonia in an oxidative deamination reaction. ABP1 activity plays an important role in the catabolism of histamine and other bioactive polyamines. The activity of recombinant human ABP1 was measured by its ability to produce hydrogen peroxide during the oxidation of histamine. The reaction was performed in 50 mM HEPES, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of ABP1 (diluted by assay buffer) to 50 µl substrate mixture of 10 uM histamine, 2 units/mL HRP and 100 µM AUR. Read at excitation and emission wavelengths of 544 nm and 590 nm (top read), respectively in kinetic mode for 5 minutes.
用法
Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA656Hu61 | 二胺氧化酶(ABP1)活性蛋白 | Cell culture; Activity Assays. |
| EPA656Hu61 | 二胺氧化酶(ABP1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA656Hu01 | 二胺氧化酶(ABP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA656Hu02 | 二胺氧化酶(ABP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA656Hu01 | 二胺氧化酶(ABP1)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAA656Hu02 | 二胺氧化酶(ABP1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA656Hu71 | 二胺氧化酶(ABP1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA656Hu22 | 二胺氧化酶(ABP1)单克隆抗体 | WB,IHC |
| MAA656Hu21 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu25 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu27 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu29 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu23 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA656Hu24 | 二胺氧化酶(ABP1)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA656Hu02 | 抗二胺氧化酶(ABP1)单克隆抗体 | Flow cytometry. |
| FAA656Hu04 | 抗二胺氧化酶(ABP1)单克隆抗体 | FCM |
| FAA656Hu06 | 抗二胺氧化酶(ABP1)单克隆抗体 | FCM |
| FAA656Hu08 | 抗二胺氧化酶(ABP1)单克隆抗体 | FCM |
| SEA656Hu | 二胺氧化酶(ABP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA656Hu | 二胺氧化酶(ABP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Applied Clay Science | Effects of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhea, intestinal permeability and proinflammatory cytokine of weanling pigs [Cas.zju: Source] |
| Phytomedicine.? | A multi-component herbal preparation, STW 5, shows anti-apoptotic effects in radiation induced intestinal mucositis in rats [Pubmed:25022208] |
| Oxid Med Cell Longev | Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model [PubMed: 26064429] |
| Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005] |
| Biochimica et Biophysica Acta (BBA)-General Subjects | Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485] |
| Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195] |
| Advances in Experimental Medicine and Biology | Serum Diamine Oxidase in Pseudoallergy in the Pediatric Population [pubmed:28804811] |
| PLoS One | Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats [pubmed:28622357] |
| Nutrients | Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron [Pubmed:29861488] |
| Scientific Reports | Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia [Pubmed:29679053] |
| British Journal of Nutrition | Postoperative symbiotic in patients with head and neck cancer: a double-blind randomised trial [Pubmed:29277158] |
| Food & Function | The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer [Pubmed:29384543] |
| 硕士论文 | LOCALIZATION OF COPPER-CONTAINING AMINE OXIDASE ENZYMES IN THE BLOOD [] |
| International Journal of Clinical and Experimental Medicine | Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease [] |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease [] |
| Acta Diabetologica | Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated mice [Pubmed: 31749050] |
| Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099] |
| FASEB JOURNAL | HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats [Pubmed: 31961020] |
| JOURNAL OF ETHNOPHARMACOLOGY | The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancer [Pubmed: 33039631] |
| Cell Death Discovery | Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in mice [Pubmed: 32821439] |
| The Journal of Nutritional Biochemistry | Effects of fucoidan on tumor prevention and gut flora [] |
| Chemical Biology & Dug Design | Design and development of novel fasudil derivatives as potent anti‐breast cancer agent that improves intestinal flora and intestinal barrier function in rats [34587363] |
| Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways [] |
| Stem Cell Research & Therapy | Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model [] |