上皮性钙黏附蛋白活性蛋白
Active E-cadherin
CDHE; CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule
- 编号APA017Hu62
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点4.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
E-Cadherin, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins. It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. As E-cadherin has the function of cell adhesion, we measure the activity of recombinant human E-cadherin by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When 5 x 104 cells/well are added to different concentrations of recombinat human E-cadherin coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MCF7 after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.23 ug/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA017Hu62 | 上皮性钙黏附蛋白活性蛋白 | Cell culture; Activity Assays. |
| RPA017Hu01 | 上皮性钙黏附蛋白重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA017Hu61 | 上皮性钙黏附蛋白活性蛋白 | Cell culture; Activity Assays. |
| EPA017Hu62 | 上皮性钙黏附蛋白真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA017Hu61 | 上皮性钙黏附蛋白真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA017Hu01 | 上皮性钙黏附蛋白多克隆抗体 | WB; IHC; ICC; IP. |
| PAA017Hu02 | 上皮性钙黏附蛋白多克隆抗体 | WB; IHC; ICC; IP. |
| LAA017Hu71 | 上皮性钙黏附蛋白多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA017Hu81 | 上皮性钙黏附蛋白多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA017Hu23 | 上皮性钙黏附蛋白单克隆抗体 | WB; IHC; ICC; IP. |
| MAA017Hu22 | 上皮性钙黏附蛋白单克隆抗体 | WB; IHC; ICC; IP. |
| MAA017Hu21 | 上皮性钙黏附蛋白单克隆抗体 | WB; IHC; ICC; IP. |
| MAA017Hu24 | 上皮性钙黏附蛋白单克隆抗体 | WB; IHC; ICC; IP. |
| SEA017Hu | 上皮性钙黏附蛋白检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA017Hu | 上皮性钙黏附蛋白检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA017Hu | 上皮性钙黏附蛋白等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| J Periodontal Res. | Novel method for proliferation of oral keratinocyte stem cells [Pubmed: 24329011] |
| The Journal of Gene Medicine | Modulation of the Dual‐Faced Effects of miR‐141 with Chitosan/miR‐141 Nanoplexes in Breast Cancer Cells [Pubmed: 31389101] |
| Cell Adhesion & Migration | BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin [Pubmed: 31957537] |
| DOKLADY BIOCHEMISTRY AND BIOPHYSICS | Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … [Pubmed: 32342301] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … [] |