谷胱甘肽S转移酶α1(GSTa1)活性蛋白
Active Glutathione S Transferase Alpha 1 (GSTa1)
GSTA1; GST2; GSTA1-1; GTH1; GST class-alpha member 1; GST HA subunit 1; GST-epsilon; Glutathione S-transferase A1, N-terminally processed
- 编号APA609Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点8.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
GSTa1 (Glutathione S-transferase a1) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant human GSTa1, mix gentliy. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human GSTa1 is >17000 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA609Hu01 | 谷胱甘肽S转移酶α1(GSTa1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA609Hu01 | 谷胱甘肽S转移酶α1(GSTa1)活性蛋白 | Cell culture; Activity Assays. |
| PAA609Hu01 | 谷胱甘肽S转移酶α1(GSTa1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA609Hu71 | 谷胱甘肽S转移酶α1(GSTa1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA609Hu81 | 谷胱甘肽S转移酶α1(GSTa1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA609Hu24 | 谷胱甘肽S转移酶α1(GSTa1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA609Hu29 | 谷胱甘肽S转移酶α1(GSTa1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA609Hu27 | 谷胱甘肽S转移酶α1(GSTa1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA609Hu21 | 谷胱甘肽S转移酶α1(GSTa1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA609Hu25 | 谷胱甘肽S转移酶α1(GSTa1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA609Hu23 | 谷胱甘肽S转移酶α1(GSTa1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA609Hu22 | 谷胱甘肽S转移酶α1(GSTa1)单克隆抗体 | WB |
| SEA609Hu | 谷胱甘肽S转移酶α1(GSTa1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA609Hu | 谷胱甘肽S转移酶α1(GSTa1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLOS ONE | Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow [PubMed: 26098421] |
| Oxidative Medicine and Cellular Longevity | Overexpression of Brg1 Alleviates Hepatic Ischemia/Reperfusion-Induced Acute Lung Injury through Antioxidative Stress Effects [pubmed:28798861] |
| Oxidative Medicine and Cellular Longevity | Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress [Pubmed: 31827710] |
| Journal of Proteomics | Complementary liver and serum protein profile in wild boars infected by the giant liver fluke Fascioloides magna using tandem mass tags quantitative approach [34298188] |