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谷胱甘肽S转移酶α3(GSTa3)活性蛋白

Active Glutathione S Transferase Alpha 3 (GSTa3)

GST-A3; GSTA3-3; GTA3; GST class-alpha member 3

  • 谷胱甘肽S转移酶α3(GSTa3)活性蛋白 产品包装(模拟)
  • 谷胱甘肽S转移酶α3(GSTa3)活性蛋白 产品包装(模拟)
  • APA671Mu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant mouse GSTa3, mix gently. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant mouse GSTa3 is >60,000 pmol/min/µg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA671Mu01 谷胱甘肽S转移酶α3(GSTa3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA671Mu01 谷胱甘肽S转移酶α3(GSTa3)活性蛋白 Cell culture; Activity Assays.
PAA671Mu01 谷胱甘肽S转移酶α3(GSTa3)多克隆抗体 WB,IHC
LAA671Mu81 谷胱甘肽S转移酶α3(GSTa3)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA671Mu71 谷胱甘肽S转移酶α3(GSTa3)多克隆抗体(生物素标记) WB; IHC; ICC.

参考文献

杂志 参考文献
Toxicon The effects of Nigella sativa seeds and thymoquinone on aflatoxin phase-2 detoxification through glutathione and glutathione-S-transferase alpha-3, and the?¡­ [33581172]
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