谷胱甘肽S转移酶μ1(GSTM1)活性蛋白
Active Glutathione S Transferase Mu 1 (GSTM1)
GST-M1; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; MU1; GST HB subunit 4
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. GSTM1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant chicken GSTM1, mix gently. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant chicken GSTM1 is >2500 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA658Ga01 | 谷胱甘肽S转移酶μ1(GSTM1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA658Ga01 | 谷胱甘肽S转移酶μ1(GSTM1)活性蛋白 | Cell culture; Activity Assays. |
| PAA658Ga01 | 谷胱甘肽S转移酶μ1(GSTM1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA658Ga71 | 谷胱甘肽S转移酶μ1(GSTM1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA658Ga81 | 谷胱甘肽S转移酶μ1(GSTM1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
参考文献
| 杂志 | 参考文献 |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| CHEMICAL SENSES | Interactions Between Odorants and Glutathione Transferases in the Human Olfactory Cleft [Pubmed: 32822470] |