谷胱甘肽S转移酶π1(GSTp)活性蛋白
Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- 编号APB090Ha01
- 物种Hamster (Chinese hamster，仓鼠) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点7.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 μl 200 mM glutathione (reduced) and 10 μl 100 mM CDNB in 980 μl 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 μl mixed substrates to 50 μl different concentrations of recombinant chinese hamster GSTP1, mix gentliy. Incubated at 37℃ for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant chinese hamster GSTp1 is >6500 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Hamster (Chinese hamster，仓鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB090Ha01 | 谷胱甘肽S转移酶π1(GSTp)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB090Ha01 | 谷胱甘肽S转移酶π1(GSTp)活性蛋白 | Cell culture; Activity Assays. |
| PAB090Ha01 | 谷胱甘肽S转移酶π1(GSTp)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB090Ha21 | 谷胱甘肽S转移酶π1(GSTp)单克隆抗体 | WB. |
| MAB090Ha22 | 谷胱甘肽S转移酶π1(GSTp)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB090Ha23 | 谷胱甘肽S转移酶π1(GSTp)单克隆抗体 | WB; IHC; ICC; IP. |
参考文献
| 杂志 | 参考文献 |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Neuroscience Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218] |
| Arabian Journal of Chemistry | Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats [] |
| International Journal of Nature and Life Sciences | The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands [] |