谷胱甘肽S转移酶π1(GSTp)活性蛋白
Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- 编号APB090Ra01
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点7.1
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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Figure. Western Blot
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Glutathione S-transferase P (GSTP1) is a member of Glutathione S-transferases (GSTs) family which play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340nm. The reaction was performed in adding 10μL 200mM glutathione (reduced) and 10μL 100mM CDNB in 980μL 100mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50μL mixed substrates to 50μL recombinant rat GSTP1, mix gentliy, read the absorbance at 340nm for 5min. One unit activity was defined as 1 μmole of CDNB conjugate per minute per 1 mg GSTP1
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB090Ra01 | 谷胱甘肽S转移酶π1(GSTp)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB090Ra01 | 谷胱甘肽S转移酶π1(GSTp)活性蛋白 | Cell culture; Activity Assays. |
| PAB090Ra01 | 谷胱甘肽S转移酶π1(GSTp)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB090Ra71 | 谷胱甘肽S转移酶π1(GSTp)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB090Ra | 谷胱甘肽S转移酶π1(GSTp)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB090Ra | 谷胱甘肽S转移酶π1(GSTp)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB090Ra01 | 谷胱甘肽S转移酶π1(GSTp)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis [Pubmed:27021019] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Neuroscience Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218] |
| Arabian Journal of Chemistry | Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats [] |
| International Journal of Nature and Life Sciences | The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands [] |