组氨酸丰富糖蛋白(HRG)活性蛋白
Active Histidine Rich Glycoprotein (HRG)
HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG
- 编号APC534Hu61
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 5% Trehalose.
- 性状冻干粉
- 纯度> 97%
- 等电点7.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
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产品包装(模拟)
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产品包装(模拟)
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Gene sequencing
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Figure. SDS-PAGE
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WB图
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Human histidine-rich glycoprotein (HRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. As HRG has the function of cell adhesion, we measure the activity of recombinant human HRG by the ability of the immobilized protein to support the adhesion of con-A activated MOLT 4 human acute lymphoblastic leukemia cells. When 1 x 105 cells/well with 7.5 ug/ml conA are added to recombinat human HRG coated plates (0.1 µg/mL with 100 µL/well), >70% cells will adhere after 2 hour incubation at 37 ℃. The adhesion of MOLT-4 after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC534Hu01 | 组氨酸丰富糖蛋白(HRG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC534Hu61 | 组氨酸丰富糖蛋白(HRG)活性蛋白 | Cell culture; Activity Assays. |
| EPC534Hu61 | 组氨酸丰富糖蛋白(HRG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC534Hu01 | 组氨酸丰富糖蛋白(HRG)多克隆抗体 | WB; IHC; ICC; IP. |
| LAC534Hu71 | 组氨酸丰富糖蛋白(HRG)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC534Hu24 | 组氨酸丰富糖蛋白(HRG)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC534Hu22 | 组氨酸丰富糖蛋白(HRG)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC534Hu21 | 组氨酸丰富糖蛋白(HRG)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC534Hu23 | 组氨酸丰富糖蛋白(HRG)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC534Hu26 | 组氨酸丰富糖蛋白(HRG)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC534Hu | 组氨酸丰富糖蛋白(HRG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC534Hu | 组氨酸丰富糖蛋白(HRG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| J. Proteome Res. | Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS [ACS: pr300330z] |
| Medical Oncology | Evaluation of histidine-rich glycoprotein tissue RNA and serum protein as novel markers for breast cancer [Springer: Source] |
| Int J Cancer | The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine‐rich glycoprotein [PubMed: 25363753] |
| Nucleic Acid Therapeutics | Pharmacokinetic and Pharmacodynamic Investigations of ION-353382, a Model Antisense Oligonucleotide: Using Alpha-2-Macroglobulin and Murinoglobulin Double-Knockout Mice [Pubmed:27031383] |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html] |
| Hypertension Research | Combination of serum histidine-rich glycoprotein and uterine artery Doppler to predict preeclampsia [Pubmed:29416108] |
| journal of histochemistry & cytochemistry | Ultrastructural Localization of Histidine-rich Glycoprotein in Skeletal Muscle Fibers: Colocalization With AMP Deaminase [Pubmed: 31880188] |
| DOKLADY BIOCHEMISTRY AND BIOPHYSICS | Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy … [Pubmed: 32342301] |