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吲哚胺2,3-双加氧酶(IDO)活性蛋白

Active Indoleamine-2,3-Dioxygenase (IDO)

CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase

  • 吲哚胺2,3-双加氧酶(IDO)活性蛋白 产品包装(模拟)
  • 吲哚胺2,3-双加氧酶(IDO)活性蛋白 产品包装(模拟)
  • 吲哚胺2,3-双加氧酶(IDO)活性蛋白 Figure . Gene Sequencing (extract)
  • APB547Hu01.jpg Figure. SDS-PAGE; Sample: Active recombinant IDO, Human.
  • 吲哚胺2,3-双加氧酶(IDO)活性蛋白 Figure. Western Blot; Sample: Recombinant IDO, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. The reaction was performed in 50 mM MES, pH 6.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of IDO (diluted by Assay Buffer) to 50 µL substrate mixture of 800 μM L-tryptophan, 9000 units/mL catalase （RPC418Hu05）, and 40 μM Methyene Blue in assay buffer with equal volume of 80 mM ascorbic acid in 0.405 M Tris, pH 8.0. The final well serves as a negative control with no IDO, replaced with 50μL assay buffer. The absorbance was read in 321 nm in kinetic mode for 5 minutes. The result indicated that recombinant human IDO can oxidize L-tryptophan, the specific activity is 10581 pmol/min/μg.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPB547Hu01 吲哚胺2,3-双加氧酶(IDO)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB547Hu01 吲哚胺2,3-双加氧酶(IDO)活性蛋白 Cell culture; Activity Assays.
PAB547Hu01 吲哚胺2,3-双加氧酶(IDO)多克隆抗体 WB; IHC; ICC; IP.
LAB547Hu71 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB547Hu81 吲哚胺2,3-双加氧酶(IDO)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB547Hu22 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 WB; IHC; ICC; IP.
MAB547Hu21 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 WB; IHC.
MAB547Hu23 吲哚胺2,3-双加氧酶(IDO)单克隆抗体 WB; IHC; ICC; IP.
FAB547Hu08 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
FAB547Hu06 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
FAB547Hu04 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
FAB547Hu02 抗吲哚胺2,3-双加氧酶(IDO)单克隆抗体 Flow cytometry.
LAB547Hu72 吲哚胺2,3-双加氧酶(IDO)单克隆抗体(生物素标记) WB; IHC; ICC.
SEB547Hu 吲哚胺2,3-双加氧酶(IDO)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB547Hu 吲哚胺2,3-双加氧酶(IDO)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PSB547Hu01 吲哚胺2,3-双加氧酶(IDO)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB547Hu01 吲哚胺2,3-双加氧酶(IDO)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Blood CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects [Pubmed: 21803856]
Clinical Transplantation Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT [Wiley: source]
Indonesian Journal of Cancer Chemoprevention Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell [Iregway: Source]
PLoS ONE CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms [PubMed: PMC3683035]
Hepatology Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma [Pubmed: 23960017]
Free Radical Biology and Medicine Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans [ScienceDirect: S0891584913006345]
Frontiers in Oncology Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation [Frontiersin:Source]
Tissue Engineering Part A Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells [Pubmed:24813640]
Immunobiology. The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered [Pubmed:24947894]
Apoptosis Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes [Pubmed:25577277]
Front Oncol Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation [PubMed: 26029659]
Acta Universitatis Tamperensis Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties [Handle: 10024]
PLoS One Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase [pubmed:27695127]
Journal of Biomedical Nanotechnology Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility [Pubmed:29883557]
Biochemistry Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells []
American Journal of Pathology Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice [Pubmed: 30980800]
international immunopharmacology Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression [Pubmed: 31176083]
Stem Cell Reports Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4 []
International Journal of?Tryptophan?Research Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡­ [33343199]
Stem Cell Reports Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4 []
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … [34802039]
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