白介素17C(IL17C)活性蛋白
Active Interleukin 17C (IL17C)
Cytokine CX2
- 编号APD347Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点8.2
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1267 ¥ 3168 ¥ 6336 ¥ 19008 ¥ 47520
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant IL17C, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to play a crucial role in innate immunity of the epithelium. IL17C binds with affinity to IL17RA. Thus, a binding ELISA assay was constructed to detect the association of rhIL17C with IL17RA. Briefly, rhIL17C were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
Figure. The binding activity of IL17C with IL17RA.
用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD347Hu01 | 白介素17C(IL17C)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD347Hu01 | 白介素17C(IL17C)活性蛋白 | Cell culture; Activity Assays. |
| PAD347Hu01 | 白介素17C(IL17C)多克隆抗体 | WB; IHC; ICC; IP. |
| MAD347Hu21 | 白介素17C(IL17C)单克隆抗体 | WB; IHC; ICC; IP. |
| SED347Hu | 白介素17C(IL17C)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCD347Hu | 白介素17C(IL17C)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMD347Hu | 白介素17C(IL17C)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Cell Mol Gastroenterol Hepatol | Modulation of Gut Microbiota Composition by Serotonin Signaling Influences Intestinal Immune Response and Susceptibility to Colitis [Pubmed: 30716420] |