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白介素7(IL7)活性蛋白

Active Interleukin 7 (IL7)

Pre-B-cell Growth Factor; Lymphopoietin-1

  • 白介素7(IL7)活性蛋白 产品包装(模拟)
  • 白介素7(IL7)活性蛋白 产品包装(模拟)
  • APA662Hu01.jpg SDS-PAGE图
  • 白介素7(IL7)活性蛋白 Figure. Western Blot; Sample: Recombinant IL7, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

IL7 (Interleukin 7) is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. The interaction between IL17 and the IL7 receptor triggers a cascade of signals important for T-cell development within the thymus and survival within the periphery. It is reported that IL-7 acts on both resting and activated T cells, including Jurkat cells. Thus, a proliferation assay of recombinant human IL7 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL7. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL7 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL7 significantly promoted cell proliferation of Jurkat cells.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA662Hu01 白介素7(IL7)活性蛋白 Cell culture; Activity Assays.
APA662Hu61 白介素7(IL7)活性蛋白 Cell culture; Activity Assays.
RPA662Hu01 白介素7(IL7)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA662Hu61 白介素7(IL7)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA662Hu01 白介素7(IL7)多克隆抗体 WB; IHC; ICC; IP.
MAA662Hu29 白介素7(IL7)单克隆抗体 WB; IHC; ICC; IP.
MAA662Hu22 白介素7(IL7)单克隆抗体 WB; IHC; ICC; IP.
MAA662Hu21 白介素7(IL7)单克隆抗体 WB; IHC; ICC; IP.
MAA662Hu23 白介素7(IL7)单克隆抗体 WB
MAA662Hu24 白介素7(IL7)单克隆抗体 WB; IHC; ICC; IP.
MAA662Hu25 白介素7(IL7)单克隆抗体 WB; IHC; ICC; IP.
MAA662Hu27 白介素7(IL7)单克隆抗体 WB; IHC; ICC; IP.
MEA662Hu 白介素7(IL7)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA662Hu 白介素7(IL7)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA662Hu 白介素7(IL7)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Experimental Gerontology Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia [Pubmed:30071285]
Cardiovascular Therapeutics Role of IL‐7 in Fusion of Rat Bone Marrow Mesenchymal Stem Cells with Cardiomyocytes in vitro and Improvement of Cardiac Function in vivo [Pubmed: 30451388]
Stem Cells and Development The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus [Pubmed: 30511904]
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