甘露糖结合蛋白(MBL)活性蛋白
Active Mannose Binding Lectin (MBL)
MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
- 编号APB480Hu02
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点5.8
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 960 ¥ 2400 ¥ 4800 ¥ 14400 ¥ 36000
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Gene sequencing
-
SDS-PAGE图
-
Figure. Western Blot;
Sample: Recombinant MBL, Human.
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
MBL2 (Mannose-binding protein C) is a calcium-dependent lectin involved in innate immune defense, which binds mannose, fucose and N-acetylglucosamine on different microorganisms, therefore results in activation of the lectin pathway of the complement system. It has been proven that MASP-2 (Mannan-binding lectin serine protease 2) forms complexes with the pattern recognition molecules MBL2, triggers the activation of the complement system. Thus, a functional binding ELISA assay was constructed to detect the association of rhMBL2 with MASP2. Briefly, rhMBL2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MASP2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MBL2 mAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50µL stop solution to the wells and read at 450nm immediately.
The binding activity of MBL2 with MASP2 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB480Hu02 | 甘露糖结合蛋白(MBL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB480Hu02 | 甘露糖结合蛋白(MBL)活性蛋白 | Cell culture; Activity Assays. |
| APB480Hu61 | 甘露糖结合蛋白(MBL)活性蛋白 | Cell culture; Activity Assays. |
| EPB480Hu61 | 甘露糖结合蛋白(MBL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB480Hu03 | 甘露糖结合蛋白(MBL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB480Hu01 | 甘露糖结合蛋白(MBL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB480Hu02 | 甘露糖结合蛋白(MBL)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB480Hu01 | 甘露糖结合蛋白(MBL)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB480Hu03 | 甘露糖结合蛋白(MBL)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB480Hu71 | 甘露糖结合蛋白(MBL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB480Hu22 | 甘露糖结合蛋白(MBL)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB480Hu | 甘露糖结合蛋白(MBL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB480Hu | 抗甘露糖结合蛋白抗体(Anti-MBL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMB480Hu | 甘露糖结合蛋白(MBL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB480Hu01 | 甘露糖结合蛋白(MBL)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| American Journal of Nephrology | Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. [Karger: 000255439] |
| Current Eye Research | Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma [Ingenta: art00007] |
| Journal of Clinical Immunology | Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511] |
| Clinical and Experimental Immunology | Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy [PubMed: PMC3406374] |
| Clin J Am Soc Nephrol. | Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN [Pubmed: 24115193] |
| ACTA TROPICA | Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis [Pubmed: 32827456] |
| PLoS One | Complement activation profile of patients with primary focal segmental glomerulosclerosis [Pubmed: 32569286] |
| LIFE SCIENCES | The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes [34242658] |