基质金属蛋白酶2(MMP2)活性蛋白
Active Matrix Metalloproteinase 2 (MMP2)
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
- 编号APA100Hu02
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 97%
- 等电点5.2
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1927 ¥ 4818 ¥ 9636 ¥ 28908 ¥ 72270
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Gene sequencing
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant MMP2, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 (500ng, 125ng, 63ng, 32ng, 16ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control.
Result 1: Gelatin hydrolysis by recombinant human MMP2 was shown in figure 1.
用法
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA100Hu62 | 基质金属蛋白酶2(MMP2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA100Hu62 | 基质金属蛋白酶2(MMP2)活性蛋白 | Cell culture; Activity Assays. |
| RPA100Hu01 | 基质金属蛋白酶2(MMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA100Hu01 | 基质金属蛋白酶2(MMP2)活性蛋白 | Cell culture; Activity Assays. |
| APA100Hu61 | 基质金属蛋白酶2(MMP2)活性蛋白 | Cell culture; Activity Assays. |
| RPA100Hu03 | 基质金属蛋白酶2(MMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA100Hu61 | 基质金属蛋白酶2(MMP2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA100Hu02 | 基质金属蛋白酶2(MMP2)活性蛋白 | Cell culture; Activity Assays. |
| RPA100Hu02 | 基质金属蛋白酶2(MMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA100Hu02 | 基质金属蛋白酶2(MMP2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA100Hu01 | 基质金属蛋白酶2(MMP2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA100Hu71 | 基质金属蛋白酶2(MMP2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA100Hu81 | 基质金属蛋白酶2(MMP2)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| FAA100Hu41 | 抗基质金属蛋白酶2(MMP2)多克隆抗体(藻红素标记) | Flow cytometry. |
| MAA100Hu27 | 基质金属蛋白酶2(MMP2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA100Hu23 | 基质金属蛋白酶2(MMP2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA100Hu26 | 基质金属蛋白酶2(MMP2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA100Hu22 | 基质金属蛋白酶2(MMP2)单克隆抗体 | WB; IHC |
| MAA100Hu21 | 基质金属蛋白酶2(MMP2)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA100Hu02 | 抗基质金属蛋白酶2(MMP2)单克隆抗体 | Flow cytometry. |
| FAA100Hu04 | 抗基质金属蛋白酶2(MMP2)单克隆抗体 | Flow cytometry. |
| FAA100Hu06 | 抗基质金属蛋白酶2(MMP2)单克隆抗体 | Flow cytometry. |
| FAA100Hu08 | 抗基质金属蛋白酶2(MMP2)单克隆抗体 | Flow cytometry. |
| SEA100Hu | 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA100Hu | 基质金属蛋白酶2(MMP2)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA100Hu | 基质金属蛋白酶2(MMP2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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