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黏病毒耐药蛋白1(MX1)活性蛋白

Active Myxovirus Resistance 1 (MX1)

IFI-78K; IFI78; MX; MxA; Interferon-Inducible Protein p78; Interferon-regulated resistance GTP-binding protein MxA

  • 黏病毒耐药蛋白1(MX1)活性蛋白 产品包装(模拟)
  • 黏病毒耐药蛋白1(MX1)活性蛋白 产品包装(模拟)
  • 黏病毒耐药蛋白1(MX1)活性蛋白 Figure. Gene Sequencing (Extract)
  • APL763Hu01.png Figure. SDS-PAGE
  • 黏病毒耐药蛋白1(MX1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of MX1 with TUBb.
Interferon-induced GTP-binding protein Mx1 is a protein that in humans is encoded by the MX1 gene. In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Besides, Tubulin Beta (TUBb) has been identified as an interactor of MX1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MX1 and recombinant human TUBb. Briefly, MX1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to TUBb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MX1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MX1 and TUBb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

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编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APL763Hu01 黏病毒耐药蛋白1(MX1)活性蛋白 Cell culture; Activity Assays.
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LML763Hu 黏病毒耐药蛋白1(MX1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PSL763Hu01 黏病毒耐药蛋白1(MX1)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSL763Hu01 黏病毒耐药蛋白1(MX1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".
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