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血小板因子4(PF4)活性蛋白

Active Platelet Factor 4 (PF4)

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

  • 血小板因子4(PF4)活性蛋白 产品包装(模拟)
  • 血小板因子4(PF4)活性蛋白 产品包装(模拟)
  • APA172Mu01.png Figure. SDS-PAGE
  • 血小板因子4(PF4)活性蛋白 Sample: Recombinant PF4, Mouse;
    Antibody: Rabbit Anti-Mouse PF4 Ab (PAA172Mu01)
    Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. To measure its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells, HUVEC cells were seeded into 96-well plates at a density of 3,000 cells/well with 2% serum standard DMEM including 1μg/mL recombinant human FGF1 and various concentrations of recombinant human PF4. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-2 hours at 37℃. Proliferation of HUVEC cells after incubation with PF4 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant human PF4 for 48h. The result was shown in Figure 2. It was obvious that PF4 significantly FGF basic-dependent proliferation of HUVEC cells. The ED50 is 3.4μg/mL.
(A)HUVEC cells cultured in DMEM with 1μg/mL FGF1, stimulated with 5μg/mL PF4 for 48h;
(B) Unstimulated HUVEC cells cultured in DMEM with 1μg/mL FGF1 for 48h.
Figure. Inhibition of HUVEC cells proliferation after stimulated with PF4.

Figure. Inhibition of FGF basic-dependent HUVEC proliferation after stimulated with PF4.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APA172Mu01 血小板因子4(PF4)活性蛋白 Cell culture; Activity Assays.
RPA172Mu01 血小板因子4(PF4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA172Mu02 血小板因子4(PF4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA172Mu01 血小板因子4(PF4)多克隆抗体 WB; IHC; ICC; IP.
PAA172Mu02 血小板因子4(PF4)多克隆抗体 WB; IHC; ICC; IP.
LAA172Mu71 血小板因子4(PF4)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA172Mu81 血小板因子4(PF4)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEA172Mu 血小板因子4(PF4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA172Mu 血小板因子4(PF4)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA172Mu 血小板因子4(PF4)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA172Mu01 血小板因子4(PF4)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic rats [PubMed: 20601223]
Basic Research in Cardiology Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170]
PLoS One Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation [PubMed: PMC3608633]
Diving and Hyperbaric Medicine Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression [Pubmed:25311322]
Journal of Functional Foods A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivo [science:S1756464616300676]
Plos one Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury [pubmed:27583400]
international journal of molecular sciences Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation. [pubmed:28272338]
Experimental and Therapeutic Medicine Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation ability [pubmed:28450960]
Reproductive Toxicology Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats [Pubmed: 30528429]
INTERNATIONAL JOURNAL OF CARDIOLOGY Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation [Pubmed: 30857843]
Available at SSRN 3335365  Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers []
journal of biotechnology Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis [Pubmed: 30927490]
Data in Brief Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation []
journal of thoracic and cardiovascular surgery Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers [Pubmed: 32812629]
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230]
Thrombosis Research Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449]
Nat Commun The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets [34099640]
Vaccine The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388]
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