太阳集团tyc5997_平台(china)官方网站

脾脏酪氨酸激酶(SYK)活性蛋白

Active Spleen Tyrosine Kinase (SYK)

p72-Syk; Spleen tyrosine kinase

  • 脾脏酪氨酸激酶(SYK)活性蛋白 产品包装(模拟)
  • 脾脏酪氨酸激酶(SYK)活性蛋白 产品包装(模拟)
  • APE275Hu01.png Figure. SDS-PAGE
  • 脾脏酪氨酸激酶(SYK)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of SYK with SIGLEC2.
Spleen Tyrosine Kinase (SYK) is a member of the SYK family of tyrosine kinases. These non-receptor cytoplasmic tyrosine kinases share a characteristic dual SH2 domain separated by a linker domain. Within B and T cells respectively, SYK and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. SYK plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. Besides, Sialic Acid Binding Ig Like Lectin 1 (SIGLEC2) has been identified as an interactor of SYK, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SYK and recombinant human SIGLEC2. Briefly, SYK were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to SIGLEC2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SYK pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SYK and SIGLEC2 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPE275Hu01 脾脏酪氨酸激酶(SYK)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APE275Hu01 脾脏酪氨酸激酶(SYK)活性蛋白 Cell culture; Activity Assays.
PAE275Hu01 脾脏酪氨酸激酶(SYK)多克隆抗体 WB,IHC
MAE275Hu21 脾脏酪氨酸激酶(SYK)单克隆抗体 WB; IHC; ICC; IP.
SEE275Hu 脾脏酪氨酸激酶(SYK)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LME275Hu 脾脏酪氨酸激酶(SYK)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Scientific Reports Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinase [Pubmed: 31949205]
留言咨询
Baidu
sogou