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干细胞因子(SCF)活性蛋白

Active Stem Cell Factor (SCF)

KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2

  • 干细胞因子(SCF)活性蛋白 产品包装(模拟)
  • 干细胞因子(SCF)活性蛋白 产品包装(模拟)
  • APA120Ca61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis and melanogenesis. SCF has been shown to  stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 104 cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37 ℃. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of SCF was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells .The ED50 for this effect is typically 0.54 ng/ml.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Canis familiaris; Canine (Dog，犬) 应用(仅供研究使用，不用于临床诊断！)
APA120Ca61 干细胞因子(SCF)活性蛋白 Cell culture; Activity Assays.
RPA120Ca01 干细胞因子(SCF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA120Ca61 干细胞因子(SCF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA120Ca01 干细胞因子(SCF)多克隆抗体 WB; IHC; ICC; IP.
MAA120Ca21 干细胞因子(SCF)单克隆抗体 WB; IHC; ICC; IP.
SEA120Ca 干细胞因子(SCF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA120Ca 干细胞因子(SCF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Archives of Gynecology and Obstetrics Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and antagonists, on follicular fluid stem cell factor and serum urocortin 1 levels on the day of oocyte retrieval [Pubmed: 23801011]
Medicine (Baltimore) An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370]
Tumor Biology Hypoxia-induced secretion of IL-13 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Molecular Neurobiology Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology. [pubmed:27730513]
Oncotarget Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy [Pubmed:29423092]
Journal of Environmental Research and Public Health Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats [Pubmed: 30974849]
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