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组织因子通道抑制因子(TFPI)活性蛋白

Active Tissue Factor Pathway Inhibitor (TFPI)

TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor

  • 组织因子通道抑制因子(TFPI)活性蛋白 产品包装(模拟)
  • 组织因子通道抑制因子(TFPI)活性蛋白 产品包装(模拟)
  • APA394Hu03.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation. It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The activity of recombinant human TFPI was measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Trypsin was diluted to 50 ug/ml in the assay buffer and 20 ul different concentrations of recombinant human TFPI (MW: 55.58 KD) was incubated with 20 ul diluted trypsin at 37 ℃ for 15 minutes. Loading 50 µL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 µL of 20 µM substrate. Include a substrate blank containing 50 µL of assay buffer and 50 µL of 20 µM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human TFPI significantly decreased trypsin activity. The inhibition IC50 was <17 nM.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA394Hu03 组织因子通道抑制因子(TFPI)活性蛋白 Cell culture; Activity Assays.
RPA394Hu02 组织因子通道抑制因子(TFPI)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA394Hu61 组织因子通道抑制因子(TFPI)活性蛋白 Cell culture; Activity Assays.
EPA394Hu61 组织因子通道抑制因子(TFPI)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA394Hu01 组织因子通道抑制因子(TFPI)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA394Hu03 组织因子通道抑制因子(TFPI)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA394Hu03 组织因子通道抑制因子(TFPI)多克隆抗体 WB; IHC; ICC; IP.
PAA394Hu01 组织因子通道抑制因子(TFPI)多克隆抗体 WB; IHC; ICC; IP.
PAA394Hu02 组织因子通道抑制因子(TFPI)多克隆抗体 WB; IHC; ICC; IP.
LAA394Hu71 组织因子通道抑制因子(TFPI)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA394Hu21 组织因子通道抑制因子(TFPI)单克隆抗体 WB; IHC; ICC; IP.
MAA394Hu26 组织因子通道抑制因子(TFPI)单克隆抗体 WB; IHC; ICC; IP.
RAA394Hu64 组织因子通道抑制因子(TFPI)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAA394Hu22 组织因子通道抑制因子(TFPI)单克隆抗体 WB; IHC; ICC; IP.
MAA394Hu23 组织因子通道抑制因子(TFPI)单克隆抗体 WB; IHC; ICC; IP.
LAA394Hu72 组织因子通道抑制因子(TFPI)单克隆抗体(生物素标记) WB; IHC; ICC.
WEA394Hu 组织因子通道抑制因子(TFPI)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA394Hu 组织因子通道抑制因子(TFPI)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA394Hu 组织因子通道抑制因子(TFPI)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA394Hu 组织因子通道抑制因子(TFPI)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA394Hu 组织因子通道抑制因子(TFPI)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PSA394Hu01 组织因子通道抑制因子(TFPI)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA394Hu01 组织因子通道抑制因子(TFPI)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
South African Journal of Child Health Tissue factor pathway inhibitor in paediatric patients with nephrotic syndrome [Sajch: 383]
the journal of immunology Cathepsin G in Experimental Tuberculosis: Relevance for Antibacterial Protection and Potential for Immunotherapy [PubMed: 26320257]
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502]
Molecular Carcinogenesis Endothelial cell‐anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in mice [PubMed: 25945811]
Cell Biology International Extracellular vesicles from malignant effusions induce tumor cell migration: inhibitory effect ofLMWH tinzaparin. [pubmed:27435911]
International Journal of Infectious Diseases Kunitz-type protease inhibitor as a vaccine candidate against schistosomiasis mansoni [pubmed:29128645]
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