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转化生长因子α(TGFa)活性蛋白

Active Transforming Growth Factor Alpha (TGFa)

ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF

  • 转化生长因子α(TGFa)活性蛋白 产品包装(模拟)
  • 转化生长因子α(TGFa)活性蛋白 产品包装(模拟)
  • 转化生长因子α(TGFa)活性蛋白 Figure . Gene Sequencing (extract)
  • APA123Hu01.jpg SDS-PAGE图
  • 转化生长因子α(TGFa)活性蛋白 Figure. Western Blot; Sample: Recombinant TGFa, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Transforming growth factor alpha (TGF-α), a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. To test the effect of TGF-α on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of TGF-α. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of 3T3 cells after incubation with TGF-α for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of TGF-α was shown in Figure 2. It was obvious that TGF-α significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 0.6198 to 8.210ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
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参考文献

杂志 参考文献
Journal of Physiology and Biochemistry Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w]
Oncology Reports A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitro [PubMed: PMC3583590]
Sci Total Environ. The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female. [pubmed:28174048]
International Journal of Biological Sciences CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 []
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets [Pubmed: 33164313]
Toll-like receptor 5-mediated signaling enhances liver regeneration in mice []
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