死亡受体5(DR5)活性蛋白
Active Death receptor 5 (DR5)
CD262; TNFRSF10B; KILLER; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9; Death receptor 5; TNF-related apoptosis-inducing ligand receptor 2; Tumor Necrosis Factor Receptor Superfamily, Member 10B
- 编号APA818Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点5.6
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1608 ¥ 4020 ¥ 8040 ¥ 24120 ¥ 60300
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产品包装(模拟)
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产品包装(模拟)
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant TNFRSF10B, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
TNFRSF10B (Tumor necrosis factor receptor superfamily member 10B) is a member of the TNF-receptor superfamily, and contains an intracellular death domain. By binding to certain ligand, this receptor transduces an apoptosis signal. A binding ELISA assay was conducted to detect the association of FAS with TNFa. Briefly, FAS were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL FAS were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FAS pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FAS and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA818Hu03 | 死亡受体5(DR5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA818Hu01 | 死亡受体5(DR5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA818Hu01 | 死亡受体5(DR5)活性蛋白 | Cell culture; Activity Assays. |
| RPA818Hu02 | 死亡受体5(DR5)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA818Hu01 | 死亡受体5(DR5)多克隆抗体 | WB,IHC,ICC/IF |
| SEA818Hu | 死亡受体5(DR5)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA818Hu | 死亡受体5(DR5)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |