肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白
Active Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)
CD120B; P75; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR2; TNFR80; P75tnfr; Tumor necrosis factor receptor 2; p80 TNF-alpha receptor; Etanercept
- 编号APB504Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点4.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1267 ¥ 3168 ¥ 6336 ¥ 19008 ¥ 47520
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure. Gene Sequencing (Extract)
-
Figure. SDS-PAGE
-
Figure. Western Blot
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) , also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Besides, Tumor Necrosis Factor Alpha (TNFa) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNFa. Briefly, TNFRSF1B were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNFa-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF1B and TNFa was shown in Figure 1, the EC50 was 0.0470~0.085 ug/ml.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPB504Hu61 | 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB504Hu01 | 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)活性蛋白 | Cell culture; Activity Assays. |
| RPB504Hu01 | 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB504Hu01 | 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)多克隆抗体 | WB; IHC; ICC; IP. |
| FAB504Hu51 | 抗肿瘤坏死因子受体超家族成员1B(TNFRSF1B)多克隆抗体(别藻蓝蛋白标记) | FCM |
| MAB504Hu21 | 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB504Hu | 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB504Hu | 肿瘤坏死因子受体超家族成员1B(TNFRSF1B)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |