肿瘤坏死因子受体超家族成员9(TNFRSF9)活性蛋白
Active Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9)
CD137; CDw137; 4-1BB; ILA; Induced By Lymphocyte Activation; T-cell antigen 4-1BB homolog
- 编号APB527Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 97%
- 等电点8.3
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1080 ¥ 2700 ¥ 5400 ¥ 16200 ¥ 40500
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure. SDS-PAGE
-
WB图
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
TNFRSF9 (Tumor necrosis factor receptor superfamily member 9) is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. A binding ELISA assay was conducted to detect the interaction of TNFRSF9 and TNFa. Briefly, recombinant human TNFRSF9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF9 and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APB527Hu01 | 肿瘤坏死因子受体超家族成员9(TNFRSF9)活性蛋白 | Cell culture; Activity Assays. |
| RPB527Hu01 | 肿瘤坏死因子受体超家族成员9(TNFRSF9)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB527Hu01 | 肿瘤坏死因子受体超家族成员9(TNFRSF9)多克隆抗体 | WB;FCM |
| FAB527Hu01 | 抗肿瘤坏死因子受体超家族成员9(TNFRSF9)多克隆抗体 | Flow cytometry. |
| LAB527Hu71 | 肿瘤坏死因子受体超家族成员9(TNFRSF9)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| RAB527Hu21 | 肿瘤坏死因子受体超家族成员9(TNFRSF9)重组抗体 | WB; IFC; IHC; IP. |
| SEB527Hu | 肿瘤坏死因子受体超家族成员9(TNFRSF9)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB527Hu | 肿瘤坏死因子受体超家族成员9(TNFRSF9)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |