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钙网蛋白(CALR)多克隆抗体

Polyclonal Antibody to Calreticulin (CALR)

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

  • 钙网蛋白(CALR)多克隆抗体 产品包装(模拟)
  • 钙网蛋白(CALR)多克隆抗体 产品包装(模拟)
  • 钙网蛋白(CALR)多克隆抗体 DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CRT Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human CRT Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human CRT Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 DAB staining on IHC-P;
    Sample: Porcine Small intestine Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human CALR Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human CALR Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human CALR Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 FITC staining on IF;
    Sample: Human MCF7 cell;
    Primary Ab: 30ug/ml Rabbit Anti-Human CALR Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAB486Hu01.jpg Figure. Western Blot; Sample: Recombinant CRT, Human.
  • 钙网蛋白(CALR)多克隆抗体 Western Blot; Sample: Human HL60 cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Human CRT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Human CRT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 Western Blot; Sample: Mouse Cerebrum lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human CRT Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 钙网蛋白(CALR)多克隆抗体 Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CALR Polyclonal Antibody (Catalog PAB486Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对CALR的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CALR。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-30μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APB486Hu61 钙网蛋白(CALR)活性蛋白 Cell culture; Activity Assays.
EPB486Hu61 钙网蛋白(CALR)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB486Hu03 钙网蛋白(CALR)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB486Hu02 钙网蛋白(CALR)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB486Hu01 钙网蛋白(CALR)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPB486Hu21 钙网蛋白(CALR)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAB486Hu02 钙网蛋白(CALR)多克隆抗体 WB; IHC; ICC; IP; FCM
PAB486Hu01 钙网蛋白(CALR)多克隆抗体 WB; IHC; ICC; IP; FCM
PAB486Hu08 钙网蛋白(CALR)多克隆抗体 WB; IHC; ICC; IP.
LAB486Hu71 钙网蛋白(CALR)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB486Hu22 钙网蛋白(CALR)单克隆抗体 WB; IHC; ICC; IP.
SEB486Hu 钙网蛋白(CALR)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB486Hu 钙网蛋白(CALR)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB486Hu 钙网蛋白(CALR)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB486Hu01 钙网蛋白(CALR)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Investigative Dermatology Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes [Pubmed: 23771121]
International Journal of Molecular Sciences The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC [Mdpi: Source]
The Journal of Maternal-Fetal & Neonatal Medicine Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes [Pubmed:26953684]
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. [pubmed:28152632]
Talanta Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling [Doi: 10.1016/j.talanta.2018.11.098]
Sci Adv Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia [Pubmed:35171673]
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