视黄醇结合蛋白4(RBP4)多克隆抗体
Polyclonal Antibody to Retinol Binding Protein 4 (RBP4)
PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma
- 编号PAA929Bo01
- 物种Bos taurus; Bovine (Cattle，牛) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA929Bo01-视黄醇结合蛋白4(RBP4)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度0.5mg/ml
- 且适物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，猪), Ovis aries; Ovine (Sheep，绵羊)
- 应用WB; IHC
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 680 ¥ 1588 ¥ 2268 ¥ 5670 ¥ 22680
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Bovine Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Bovine RBP4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Bovine Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Bovine Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Bovine RBP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Bovine Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Bovine RBP4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant RBP4, Bovine.
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Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: RBP4 knockout HepG2 cell lysate;
Predicted MW: 21kDa
Observed MW: 21kDa
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Bovine Kidney lysate;
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Bovine Serum;
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Caprine Plasma;
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Bovine Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Serum
Primary Ab: 1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Bovine Liver lysate; Lane2: Bovine Serum; Lane3: Bovine Kidney lysate; Lane4: Porcine Serum;
Primary Ab: 0.1µg/ml Rabbit Anti-Bovine RBP4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对RBP4的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别RBP4。
用法
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Bos taurus; Bovine (Cattle，牛) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA929Bo01 | 视黄醇结合蛋白4(RBP4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA929Bo02 | 视黄醇结合蛋白4(RBP4)活性蛋白 | Cell culture; Activity Assays. |
| PAA929Bo01 | 视黄醇结合蛋白4(RBP4)多克隆抗体 | WB; IHC |
| PAA929Bo02 | 视黄醇结合蛋白4(RBP4)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA929Bo81 | 视黄醇结合蛋白4(RBP4)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA929Bo71 | 视黄醇结合蛋白4(RBP4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA929Bo21 | 视黄醇结合蛋白4(RBP4)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA929Bo | 视黄醇结合蛋白4(RBP4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA929Bo | 视黄醇结合蛋白4(RBP4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA929Bo01 | 视黄醇结合蛋白4(RBP4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Diabetes Technology & Therapeutics | Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078] |
| Journal of Pharmaceutical and Biomedical Analysis | Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 [PubMed: 21130593] |
| Evid Based Complement Alternat Med | Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats [PubMed: PMC3368504] |
| Journal of Endocrinology | Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles [Pubmed:24501381] |
| J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061] |
| Peptides | Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats [Pubmed:25445608] |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126] |
| Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173] |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazone [Pubmed:27446311] |
| Experimental and Therapeutic Medicine | Expression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone. [pubmed:27446311] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| BMC Cancer | Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis [Pubmed:29386009] |
| Nutrition & Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416] |
| Molecular Medicine Reports | RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway [Pubmed:30015949] |
| Relationship between retinol and risk of diabetic retinopathy: a case-control study [] | |
| Scientific Reports | Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle [Pubmed: 31147589] |
| Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals [] | |
| Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma [] | |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861] |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507] |
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