脂联素(ADPN)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Adiponectin (ADPN)
GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1
- 编号SCA605Ga
- 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围10.97-8,000pg/mL
- 灵敏度最小可检测剂量小于等于4.73pg/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3448 ¥ 4926 ¥ 22167 ¥ 41871 ¥ 344820
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测脂联素(ADPN)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的脂联素(ADPN)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 89-104 | 98 |
| EDTA plasma(n=5) | 86-93 | 89 |
| heparin plasma(n=5) | 92-101 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的脂联素(ADPN)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中脂联素(ADPN)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-101% | 78-104% | 83-97% | 79-94% |
| EDTA plasma(n=5) | 83-91% | 78-94% | 93-101% | 94-104% |
| heparin plasma(n=5) | 93-101% | 80-97% | 87-94% | 84-92% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。
实验原理
将脂联素(ADPN)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的脂联素(ADPN)与连接于固相载体上的抗体结合，然后加入生物素化的脂联素(ADPN)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的脂联素(ADPN)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA605Ga01 | 脂联素(ADPN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA605Ga01 | 脂联素(ADPN)活性蛋白 | Cell culture; Activity Assays. |
| PAA605Ga01 | 脂联素(ADPN)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA605Ga21 | 脂联素(ADPN)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA605Ga | 脂联素(ADPN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA605Ga | 脂联素(ADPN)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA605Ga | 脂联素(ADPN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| European Journal of Pharmacology | Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet [PubMed: 20869961] |
| BioMed Research International | Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats [PubMed: PMC3591142] |
| PLoS One | CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-Antibodies [PubMed: PMC3297623] |
| Molecular and Cellular Endocrinology | Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs [ScienceDirect: S0303720712004194] |
| British Journal of Nutrition | n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs [Pubmed: 23947577] |
| International Urology Nephrology | Perirenal fat associated with microalbuminuria in obese rats [Pubmed: 24526332] |
| Int J Mol Sci. | Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. [Pubmed: 24736781] |
| Mol Biol Rep. | Adiponectin decreases lipids deposition by p38 MAPK/ATF2 signaling pathway in muscle of broilers. [Pubmed: 24178343] |
| Endocr J. | CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658] |
| Iran J Basic Med Sci | Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats [Pubmed:Pmc4046234] |
| Biometals.? | Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats [Pubmed:24519243] |
| Poult Sci.? | Effect of perches on liver health of hens [Pubmed:24812236] |
| Gen Comp Endocrinol. | The effect of recombinant adeno-associated virus-adiponectin (rAAV2/1-Acrp30) on glycolipid dysmetabolism and liver morphology in diabetic rats [Pubmed:25019654] |
| Journal of Trace Elements in Medicine and Biology | Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar rats [Pubmed:25194956] |
| J Endocrinol | Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061] |
| Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126] |
| Int J Clin Exp Pathol | Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway [PubMed: 26617802] |
| Cell Physiol Biochem | Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway [PubMed: 26160485] |
| Life Sciences | Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet [PubMed: 26188592] |
| MEDICAL RESEARCH JOURNAL | Relation between afamin level and some inflammatory markers in obese children [Mrjnrc: Abstract] |
| Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes. [Pubmed:26896925] | |
| Technologia Alimentaria | Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats [abstract:10] |
| Obes Surg. | Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. [Pubmed:27185175] |
| Acta Histochemica | Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234] |
| Adv Biomed Res. | Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats [pmc:PMC4817393] |
| Biological Trace Element Research | Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats [article:s12011-016-0777-1] |
| Journal of Functional Foods | Antiobesity and antihyperlipidaemic effects of Yan-Sheng-Yin in animals and humans [science:S1756464616300585] |
| J Bone Miner Res | Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats [Pubmed:26175082] |
| Journal of Molecular and Cellular Cardiology | Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice [pubmed:27638193] |
| Journal of Diabetes Research | Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose [pubmed:27127795] |
| Asian-Australasian Journal of Animal Sciences | Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits [pubmed:27004817] |
| Biomedical and Pharmacology Journal | Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- … [vol10no1] |
| IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES | Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats [pubmed:29147484] |
| Evidence-Based Complementary and Alternative Medicine | BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway [journals:1758383] |
| Oxidative Medicine and Cellular Longevity | Differential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesis [journals:3405089] |
| Iranian Journal of Pharmaceutical Research | Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance [pubmed:29201096] |
| Biomedical & Pharmacology Journal | Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control Study [DOI:10.13005/bpj/1123] |
| Oncotarget | FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway [pubmed:28515350] |
| Diabetes & metabolism | Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? [pubmed:28499696] |
| Frontiers in Endocrinology | Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … [Pubmed:29755407] |
| Food & Function | Calycosin directly improves perivascular adipose tissue dysfunction by upregulating the adiponectin/AMPK/eNOS pathway in obese mice [Pubmed:29595858] |
| Evidence-based Complementary and Alternative Medicine | Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … [Pubmed:29619063] |
| Medicine (Baltimore) | Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity [Pubmed:29953010] |
| Veterinary immunology and immunopathology | Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemia [Pubmed:30078593] |
| Hematology | Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? [Pubmed:29318945] |
| International Journal of Gerontology | Aging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats [10.1016:j.ijge.2018.01.002] |
| Oncotarget | Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … [Pubmed:29484120] |
| J Bas Res Med Sci | Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats [article-1-352-en.pdf] |
| Iraq Medical Journal | Adiponectin may be used as a marker in prediction of diabetic retinopathy patients [JOCMS:Source] |
| Food Science and Technology Research | Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells [Doi: 10.3136/fstr.24.903] |
| Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783] |
| Nutrients | Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet [Pubmed: 30400147] |
| Progress in Nutrition | Effect of fermented food consumption on biochemical parameters and adipokines levels [Doi: 0.23751/pn.v20i4.7374] |
| Biology of Reproduction | Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway [Pubmed: 30496353] |
| Nutrition in the Prevention and Treatment of Abdominal Obesity | Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig [] |
| Evidence-Based Complementary and Alternative Medicine | BuShenKangShuai Tablet Alleviates Hepatic Steatosis via Improving Liver Adiponectin Resistance in ApoE-/-Mice [] |
| Life Sciences | Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in rats [Pubmed: 30826497] |
| Arteriosclerosis, Thrombosis, and Vascular Biology | Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In Vivo [Pubmed: 30816801] |
| Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP … [Pubmed: 31003008] | |
| Gene | Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension [Pubmed: 30940525] |
| 中国药理学通报 | 冷暴露对高脂饮食小鼠脂肪棕色化的影响 [] |
| Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights … [] |
| Proceedings of the Royal Society B: Biological Sciences | Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens [Pubmed: 31506054] |
| Cytokine | Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus [Pubmed: 31514105] |
| Food & Function | Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice [Pubmed: 31670366] |
| journal of ethnopharmacology | Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model [Pubmed: 31756448] |
| Annals of Nutrition and Metabolism | Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity [Pubmed: 31775140] |
| BMC Endocrine Disorders | The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561] |
| Journal of Hazardous Materials | Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice [Pubmed: 31796355] |
| Experimental and Clinical Endocrinology & Diabetes | HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic Syndrome [Pubmed: 32189318] |
| JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Cerasus humilis cherry polyphenol reduces high-fat diet-induced obesity in C57BL/6 mice by mitigating fat deposition, inflammation, and oxidation [Pubmed: 32227855] |
| Nature Communications | AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axis [Pubmed: 33199780] |
| Metabolites | Adipocytokines and Metabolic Syndrome in Patients with Schizophrenia [Pubmed: 33066473] |
| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789] |
| aging | Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats [Pubmed: 32970613] |
| Diabetes Metab Syndr Obes | Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese Population [Pubmed: 32904655] |
| DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via … [Pubmed: 32880686] |
| J Endocrinol Invest | Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … [Pubmed: 32816247] |
| JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity [Pubmed: 32633953] |
| J Endocrinol Invest | Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats [Pubmed: 32588381] |
| ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY | The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients [Pubmed: 32569480] |
| Diabetes | Exercise-Induced Increases in Insulin Sensitivity After Bariatric surgery are Mediated by Muscle Extracellular Matrix Remodeling [Pubmed: 32409493] |
| Journal of Cellular and Molecular Medicine | Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats [Pubmed: 32395890] |
| The Egyptian Journal of Chest Diseases and Tuberculosis | Study of serum adiponectin and resistin in patients with obstructive sleep apnea [] |
| Biosciences Biotechnology Research Asia | Association of+ 10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus [] |
| Computational Biology and Chemistry | In-silico therapeutic investigations of arjunic acid and arjungenin as an FXR agonist and validation in 3T3-L1 adipocytes [] |
| Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861] |
| Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274] |
| Cell Metab | TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease [34107313] |
| Biomedical and Environmental Sciences | Decreased Plasma MANF Levels are Associated with Type 2 Diabetes [33766221] |
| International Journal of Endocrinology | Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡ [34035808] |
| International Journal of Pharmacy and Biological Sciences | Anti-Obesity Efficacy of Selected Plants Against Progesterone Induced Obesity [] |
| Journal of Functional Foods | Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice [] |
| Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women [] |
| PPAR Research | Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment … [34306045] |
| Br J Nutr | The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study [34348809] |
| Phytomedicine | Smilax china L. flavonoid alleviates HFHS-induced inflammation by regulating the gut-liver axis in mice [34561124] |
| Biofactors | Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat [34767670] |
| Kaohsiung J Med Sci | Associations of imbalance of intestinal flora with severity of disease, inflammatory factors, adiponectin, and vascular endothelial function of hypertension patients [34672426] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| Animals | Effects of Obesity on Adiponectin System Skin Expression in Dogs: A Comparative Study [34438765] |
| Cell Death & Disease | Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistance [Pubmed:35347118] |
| Diabetes Metabolic Syndrome and Obesity-Targets and Therapy | Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India [Pubmed:35717896] |
| BioMed Research International | Adiponectin Levels Are Associated with White Matter Lesions (WMLs) and Cognitive Impairment [Pubmed:35087911] |
| High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients [] | |
| Scientific Reports | SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions. [] |
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