载脂蛋白A1(APOA1)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Apolipoprotein A1 (APOA1)
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
- 编号SCA519Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围1.37-1,000ng/mL
- 灵敏度最小可检测剂量小于等于0.68ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3528 ¥ 5040 ¥ 22680 ¥ 42840 ¥ 352800
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测载脂蛋白A1(APOA1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的载脂蛋白A1(APOA1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-96 | 93 |
| EDTA plasma(n=5) | 81-97 | 87 |
| heparin plasma(n=5) | 95-102 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的载脂蛋白A1(APOA1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中载脂蛋白A1(APOA1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-97% | 90-104% | 97-105% | 99-105% |
| EDTA plasma(n=5) | 95-102% | 80-93% | 78-96% | 98-105% |
| heparin plasma(n=5) | 79-88% | 81-103% | 98-105% | 89-97% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。
实验原理
将载脂蛋白A1(APOA1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的载脂蛋白A1(APOA1)与连接于固相载体上的抗体结合，然后加入生物素化的载脂蛋白A1(APOA1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的载脂蛋白A1(APOA1)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA519Hu01 | 载脂蛋白A1(APOA1)活性蛋白 | Cell culture; Activity Assays. |
| RPA519Hu01 | 载脂蛋白A1(APOA1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA519Hu61 | 载脂蛋白A1(APOA1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA519Hu01 | 载脂蛋白A1(APOA1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA519Hu71 | 载脂蛋白A1(APOA1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA519Hu21 | 载脂蛋白A1(APOA1)单克隆抗体 | WB; IHC |
| MAA519Hu24 | 载脂蛋白A1(APOA1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA519Hu26 | 载脂蛋白A1(APOA1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA519Hu23 | 载脂蛋白A1(APOA1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA519Hu25 | 载脂蛋白A1(APOA1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA519Hu29 | 载脂蛋白A1(APOA1)单克隆抗体 | WB; IHC; ICC; IP. |
| RAA519Hu21 | 载脂蛋白A1(APOA1)重组抗体 | WB; IHC; ICC. |
| RAA519Hu27 | 载脂蛋白A1(APOA1)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA519Hu22 | 载脂蛋白A1(APOA1)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA519Hu02 | 抗载脂蛋白A1(APOA1)单克隆抗体 | Flow cytometry. |
| LAA519Hu76 | 载脂蛋白A1(APOA1)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA519Hu | 载脂蛋白A1(APOA1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA519Hu | 抗载脂蛋白A1抗体(Anti-APOA1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA519Hu | 载脂蛋白A1(APOA1)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA519Hu | 载脂蛋白A1(APOA1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA519Hu01 | 载脂蛋白A1(APOA1)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA519Hu01 | 载脂蛋白A1(APOA1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| European Journal of Applied Physiology | Endurance training enhances ABCA1 expression in rat small intestine [PubMed: 19629515] |
| Official Journal of the INWR | Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. [Wrestling: source] |
| Biochemical and Biophysical Research Communications | Treadmill exercise enhances ABCA1 expression in rat liver [ScienceDirect: S0006291X0701488X] |
| World Journal of Sport Sciences | The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1 [Idosi: source] |
| Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ] |
| Biomarkers | The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry study [PubMed: 22458349] |
| Journal of the American Heart Association | MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice [PubMed: PMC3540673] |
| PLoS ONE | Apolipoprotein AI Is a Potential Mediator of Remote Ischemic Preconditioning [Plosone: Source] |
| Molecular Genetics and Metabolism | DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levels [Pubmed: 23920041] |
| Endocrinology | Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice [Pubmed: 24684302] |
| Molecular Genetics and Metabolism Reports | Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia [Sciencedirect:S2214426915000026] |
| J Psychopharmacol | Anti-atherogenic properties of high-density lipoproteins in psychiatric patients before and after two months of atypical anti-psychotic therapy [PubMed: 26253619] |
| Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
| Pharmacol Res Perspect | Efficacy of tomato concentrates in mouse models of dyslipidemia and cancer [PubMed: 26171234] |
| Clin Exp Nephrol | Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173] |
| Oncogene | Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis [PubMed: 26279300] |
| Oncogene | Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesis [Pubmed:26279300] |
| Allergy | Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis. [pubmed:28378321] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| 11 | High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signaling [pubmed:27866837] |
| Biomedical Science Letters | Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice [10.15616/BSL.2017.23.2.80] |
| ACS Biomaterials Science & Engineering | Enhanced Antiatherosclerotic Efficacy of Statin-Loaded Reconstituted High-Density Lipoprotein via Ganglioside GM1 Modification [abs:10.1021] |
| Food Research International | Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541] |
| Journal of The Science of Food and Agriculture | Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773] |
| Toxicon | A membrane disrupting toxin from wasp venom underlies the molecular mechanism of tissue damage [Pubmed:29654869] |
| European Journal of Pharmacology | The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981] |
| European Journal of Preventive Cardiology | High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats [Pubmed: 31718266] |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
| VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| Cardiovasc Toxicol | Interplay of Obesity, Ethanol, and Contaminant Mixture on Clinical Profiles of Cardiovascular and Metabolic Diseases: Evidence from an Animal Study [Pubmed:35429258] |
| Peptides | Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor [Pubmed:35660638] |
| Journal of Functional Foods | Castanea mollissima shell polyphenols regulate JAK2 and PPARγ expression to suppress inflammation and lipid accumulation by inhibiting M1 macrophages … [] |
留言咨询