皮质醇(Cor)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Cortisol (Cor)
Hydrocortisone
- 编号CCA462Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围5.86-1,500ng/mL
- 灵敏度最小可检测剂量小于等于2.37ng/mL.
- 样本类型Serum, plasma, urine and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3427 ¥ 4896 ¥ 22032 ¥ 41616 ¥ 342720
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测皮质醇(Cor)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的皮质醇(Cor)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-101 | 92 |
| EDTA plasma(n=5) | 89-101 | 93 |
| heparin plasma(n=5) | 78-91 | 86 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的皮质醇(Cor)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中皮质醇(Cor)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-99% | 81-101% | 96-104% | 86-94% |
| EDTA plasma(n=5) | 84-92% | 86-94% | 98-105% | 80-104% |
| heparin plasma(n=5) | 78-91% | 95-102% | 80-95% | 81-95% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分钟；
7. 读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将皮质醇(Cor)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原（标准品或样本），待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的皮质醇(Cor)呈负相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA462Ge11 | 皮质醇(Cor)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA462Ge21 | 皮质醇(Cor)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA462Ge01 | 皮质醇(Cor)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA462Ge02 | 皮质醇(Cor)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA462Ge81 | 皮质醇(Cor)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA462Ge71 | 皮质醇(Cor)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA462Ge21 | 皮质醇(Cor)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA462Ge22 | 皮质醇(Cor)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MEA462Ge | 皮质醇(Cor)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA462Ge | 皮质醇(Cor)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA462Ge | 皮质醇(Cor)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA462Ge | 皮质醇(Cor)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA462Ge | 皮质醇(Cor)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA462Ge | 皮质醇(Cor)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA462Ge11 | 皮质醇(Cor)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Research in Veterinary Science | Use of infrared thermography to assess the influence of high environmental temperature on rabbits [ScienceDirect: S0034528813001380] |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han Chinese [Pubmed:24124699] |
| Theriogenology | Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit model [Pubmed:25442388] |
| Animal Production Science | Effect of high temperatures on breeding rabbit behaviour [Publish:Source] |
| JBCD | Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students [Index.Php: Jbcd] |
| International Journal of Immunopathology & Pharmacology | Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638] |
| IET Micro & Nano Letters | Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chip [xpls:7393900] |
| Optoelectronics Letters | Fabrication and characterization of SPR chips with the modified bovine serum albumin [article:10.1007] |
| Journal of Dairy Science | Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503] |
| Acta Veterinaria Hungarica | Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation [acve-2017-0006] |
| BMC Veterinary Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … [Pubmed:29540178] |
| Indian Journal of Animal Sciences | Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition [:] |
| Biological Rhythm Research | Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation … [] |
| International Journal of Current Microbiology and Applied Sciences | Physiological Performance of Crossbred Cattle Calves (Karan Fries) under Different Housing Conditions during Different Seasons [] |
| Journal of Animal & Plant Sciences | A study on certain blood biomarkers of pregnant Cholistani cows. [] |
| Animals | The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot Study [Pubmed: 30866503] |
| Medicine | Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexia [Pubmed: 32028434] |
| MICROBIAL PATHOGENESIS | Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirus [Pubmed: 32926999] |
| AQUACULTURE RESEARCH | Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition [] |
| AQUACULTURE RESEARCH | Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense [] |
| Anti-stress activity of some plants extracts of the North Caucasus flora [] | |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| Parasit Vectors | Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle [33722267] |
| J Am Heart Assoc | Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest [33960200] |
| Animals | The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets [33435211] |
| Preventive Veterinary Medicine | Stress response after race and endurance training sessions and competitions in Arabian horses [33497894] |
| Current Psychology | Associations of Chinese social face with cortisol level and glucocorticoid receptor gene [] |
| Orexin-A/OX1 Receptor Regulates Autophagy, Mitochondrial Autophagy, and Cortisol Secretion in Human Adrenocortical Cells Through the mTORC1/P70s6k1 … [] |