多巴胺(DA)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Dopamine (DA)
2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride
- 编号CCA851Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围3.9-1,000pg/mL
- 灵敏度最小可检测剂量小于等于1.5pg/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3427 ¥ 4896 ¥ 22032 ¥ 41616 ¥ 342720
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测多巴胺(DA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的多巴胺(DA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-93 | 89 |
| EDTA plasma(n=5) | 79-103 | 87 |
| heparin plasma(n=5) | 93-101 | 96 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的多巴胺(DA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中多巴胺(DA)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 97-105% | 86-101% | 78-97% |
| EDTA plasma(n=5) | 80-91% | 97-104% | 80-97% | 93-101% |
| heparin plasma(n=5) | 84-103% | 84-94% | 84-92% | 81-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分钟；
7. 读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将多巴胺(DA)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原（标准品或样本），待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的多巴胺(DA)呈负相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA851Ge41 | 多巴胺(DA)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA851Ge11 | 多巴胺(DA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA851Ge21 | 多巴胺(DA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA851Ge01 | 多巴胺(DA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA851Ge71 | 多巴胺(DA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA851Ge81 | 多巴胺(DA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA851Ge21 | 多巴胺(DA)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MEA851Ge | 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA851Ge | 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA851Ge | 多巴胺(DA)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA851Ge | 多巴胺(DA)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA851Ge | 多巴胺(DA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA851Ge11 | 多巴胺(DA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Renal Failure | Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients [Pubmed: 23530612] |
| Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462] |
| Molecular Neurobiology | Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations [Pubmed:25526861] |
| Cardiology. | Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults [Pubmed:25824645] |
| Eur J Pharmacol | Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activity [PubMed: 25843409] |
| Neuropsychiatr Dis Treat | Catha edulis chewing effects on treatment of paranoid schizophrenic patients [PubMed: 25926735] |
| Journal of Neurochemistry | Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways [PubMed: 25752913] |
| Beni-Suef University Journal of Basic and Applied Sciences | Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair test [science:S2314853516300166] |
| Neuroscience | Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in rats [pubmed:27365173] |
| Metab Brain Dis. | Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes. [pubmed:27488111] |
| Nutrition | Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats [S0899900716302350] |
| Nutrition | Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats [pubmed:28241996] |
| Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats [article_1486_c0fa9f8afe5f70b407e7195bcfd55c70] | |
| Behavioural Brain Research | High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in rats [Pubmed:29842916] |
| Toxicology Reports | Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … [Pubmed:29854627] |
| Molecular and Cellular Biochemistry | Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes [Pubmed:28948423] |
| Physiology & Behavior | Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … [Pubmed:29410018] |
| Behavioural Brain Research | Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats [Pubmed: 30423388] |
| Indian Journal of Ophthalmology | Dopamine levels in human tear fluid [Pubmed: 30574889] |
| Journal of Taibah University for Science | Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles [] |
| Metabolic Brain Disease | Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brain [Pubmed: 31134480] |
| Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome [] | |
| Neurotoxicity of Mn3O4 nanoparticles: Apoptosis and dopaminergic neurons damage pathway [Pubmed: 31740235] | |
| Neurochemistry International | Polydatin protects SH-SY5Y in models of Parkinson's disease by promoting Atg5-mediated but parkin-independent autophagy [Pubmed: 31926197] |
| Neurochemistry International | Iron overload induced by IRP2 gene knockout aggravates symptoms of Parkinson's disease [Pubmed: 31904393] |
| Journal of neural transmission (Vienna, Austria : 1996) | Neurotransmitter system aberrations in patients with drug addiction [Pubmed: 32804296] |
| Acupunct Med | Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia [Pubmed: 32517478] |
| Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine [] | |
| South African Journal of Botany | Neuroprotective effects of pulicaria undulata essential oil in rotenone model of parkinson's disease in rats: Insights into its anti-inflammatory and anti-oxidant effects [] |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990] |
| Neural Regeneration Research | Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6?¡ [33433498] |
| International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ [33762821] |
| CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993] |
| PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster [34654742] |
| ACS Chem Neurosci | Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson's Disease [34197084] |
| Exp Brain Res | Effects of early life stress during stress hyporesponsive period (SHRP) on anxiety and curiosity in adolescent rats [Pubmed:35141770] |
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