成纤维细胞生长因子21(FGF21)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Fibroblast Growth Factor 21 (FGF21)
- 编号CCC918Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围1.17-300pg/mL
- 灵敏度最小可检测剂量小于等于0.43pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3830 ¥ 5472 ¥ 24624 ¥ 46512 ¥ 383040
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测成纤维细胞生长因子21(FGF21)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的成纤维细胞生长因子21(FGF21)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 95-104 | 101 |
| heparin plasma(n=5) | 91-98 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的成纤维细胞生长因子21(FGF21)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中成纤维细胞生长因子21(FGF21)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 79-94% | 78-95% | 80-88% |
| EDTA plasma(n=5) | 89-101% | 80-102% | 78-104% | 79-99% |
| heparin plasma(n=5) | 97-105% | 98-105% | 79-89% | 83-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分钟；
7. 读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将成纤维细胞生长因子21(FGF21)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原（标准品或样本），待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的成纤维细胞生长因子21(FGF21)呈负相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APC918Ra01 | 成纤维细胞生长因子21(FGF21)活性蛋白 | Cell culture; Activity Assays. |
| RPC918Ra02 | 成纤维细胞生长因子21(FGF21)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC918Ra61 | 成纤维细胞生长因子21(FGF21)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC918Ra61 | 成纤维细胞生长因子21(FGF21)活性蛋白 | Cell culture; Activity Assays. |
| RPC918Ra01 | 成纤维细胞生长因子21(FGF21)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC918Ra02 | 成纤维细胞生长因子21(FGF21)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC918Ra01 | 成纤维细胞生长因子21(FGF21)多克隆抗体 | WB; IHC; ICC; IP. |
| MAC918Ra21 | 成纤维细胞生长因子21(FGF21)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC918Ra | 成纤维细胞生长因子21(FGF21)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEC918Ra | 成纤维细胞生长因子21(FGF21)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEC918Ra | 成纤维细胞生长因子21(FGF21)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCC918Ra | 成纤维细胞生长因子21(FGF21)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC918Ra | 成纤维细胞生长因子21(FGF21)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PGC918Ra01 | 成纤维细胞生长因子21(FGF21)引物对 | PCR |
参考文献
| 杂志 | 参考文献 |
| Lipids in Health and Disease | Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs [Lipidworld: source] |
| Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression [Endocrine:Source] |
| Biochemical and Biophysical Research Communications | Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH [Pubmed:25449271] |
| Free Radic Biol Med | Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway [PubMed: 25843656] |
| Journal of diabetes and its complications | Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. [pubmed:27916484] |
| American journal of the medical sciences | Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus [pubmed:29078839] |
| Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
| Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим … [] | |
| Physiol Res | Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease. [Pubmed: 32469231] |
| Chinese Journal of Osteoporosis | 2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究. [] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| Nutrients | The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose [] |
| Medicine (Baltimore) | Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort [34918690] |
| Annals of Agricultural and Environmental Medicine | Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis [34969228] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … [Pubmed:35212074] |
| Lab Invest | Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injury [Pubmed:35488034] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … [Pubmed:35585377] |
| High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients [] |