P选择素(SELP)检测试剂盒(化学发光免疫分析法)
CLIA Kit for P-Selectin (SELP)
CD62P; Selectin, Platelet; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane
- 编号SCA569Hu
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围13.7-10,000pg/mL
- 灵敏度最小可检测剂量小于等于4.5pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
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特异性
本试剂盒用于检测P选择素(SELP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的P选择素(SELP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 89-102 | 94 |
EDTA plasma(n=5) | 78-93 | 89 |
heparin plasma(n=5) | 80-99 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的P选择素(SELP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中P选择素(SELP)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-101% | 79-93% | 95-104% | 92-101% |
EDTA plasma(n=5) | 95-104% | 98-105% | 78-104% | 96-105% |
heparin plasma(n=5) | 98-105% | 96-104% | 97-105% | 88-95% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。
实验原理
将P选择素(SELP)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的P选择素(SELP)与连接于固相载体上的抗体结合,然后加入生物素化的P选择素(SELP)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的P选择素(SELP)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA569Hu02 | P选择素(SELP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA569Hu61 | P选择素(SELP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA569Hu61 | P选择素(SELP)活性蛋白 | Cell culture; Activity Assays. |
RPA569Hu01 | P选择素(SELP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA569Hu01 | P选择素(SELP)多克隆抗体 | WB; IHC; ICC; IP. |
PAA569Hu02 | P选择素(SELP)多克隆抗体 | WB; IHC; ICC; IP. |
LAA569Hu71 | P选择素(SELP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA569Hu81 | P选择素(SELP)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAA569Hu24 | P选择素(SELP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA569Hu21 | P选择素(SELP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA569Hu22 | P选择素(SELP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA569Hu23 | P选择素(SELP)单克隆抗体 | WB; IHC; ICC; IP. |
SEA569Hu | P选择素(SELP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA569Hu | P选择素(SELP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA569Hu | P选择素(SELP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Journalof Vascular Research | Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators [Karger: 000257340] |
Basic Research in Cardiology | Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation [PubMed: 22899170] |
BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
Materials Science and Engineering: C | MRI-compatible Nb–60Ta–2Zr alloy used for vascular stents: Haemocompatibility and its correlation with protein adsorption [Pubmed:25063132] |
Blood Coagul Fibrinolysis.? | Chemical sympathectomy attenuates inflammation, glycocalyx shedding and coagulation disorders in rats with acute traumatic coagulopathy. [Pubmed:25325345] |
Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261] |
Endocrine | Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998] |
Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275] |
Nutrition & Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835] |
Available at SSRN 3335365 | Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers [] |
Ecotoxicology and Environmental Safety | Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P [] |
Thrombosis Research | Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices [Pubmed: 31838449] |
JOURNAL OF CONTROLLED RELEASE | Polycaprolactone vascular graft with epigallocatechin gallate embedded sandwiched layer-by-layer functionalization for enhanced antithrombogenicity and anti … [Pubmed: 31982435] |
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice [Pubmed: 32377309] |
blood advances | The binding of autotaxin to integrins mediates hyperhomocysteinemia-potentiated platelet activation and thrombosis [34559203] |
Vaccine | The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats [Pubmed:35183388] |
Int J Biol Macromol | The inhibition of TRIM35-mediated TIGAR ubiquitination enhances mitochondrial fusion and alleviates renal ischemia-reperfusion injury [Pubmed:35421414] |
Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |