Toll样受体4(TLR4)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Toll Like Receptor 4 (TLR4)
CD284; TOLL; HToll
- 编号SCA753Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围13.7-10,000pg/mL
- 灵敏度最小可检测剂量小于等于5.5pg/mL.
- 样本类型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3629 ¥ 5184 ¥ 23328 ¥ 44064 ¥ 362880
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Toll样受体4(TLR4)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。
实验原理
将Toll样受体4(TLR4)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Toll样受体4(TLR4)与连接于固相载体上的抗体结合，然后加入生物素化的Toll样受体4(TLR4)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的Toll样受体4(TLR4)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA753Mu01 | Toll样受体4(TLR4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA753Mu02 | Toll样受体4(TLR4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA753Mu02 | Toll样受体4(TLR4)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA753Mu01 | Toll样受体4(TLR4)多克隆抗体 | WB |
| LAA753Mu81 | Toll样受体4(TLR4)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA753Mu71 | Toll样受体4(TLR4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA753Mu | Toll样受体4(TLR4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA753Mu | Toll样受体4(TLR4)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA753Mu | Toll样受体4(TLR4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA753Mu01 | Toll样受体4(TLR4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions [CMJ: 2010823483999506297] |
| Journal of Maternal-Fetal and Neonatal Medicine | Amniotic fluid soluble Toll-like receptor 4 in pregnancies complicated by preterm prelabor rupture of the membranes [Informa: 14767058.2011.626821] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
| Clin Res Hepatol Gastroenterol. | The association between precancerous gastric lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag A status [Pubmed: 23137754] |
| Journal of Clinical Microbiology | Toll-Like Receptor 2 in Serum: a Potential Diagnostic Marker of Prosthetic Joint Infection? [Pubmed: 24478497] |
| Surgical Endoscopy | CO2 pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model. [Pubmed: 24442682] |
| Experimental and therapeutic medicine | Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin [Pubmed: 24223644] |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835] |
| Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology | Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination [Pubmed:25248345] |
| Molecular and Cellular Biochemistry | Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide [Pubmed:25318610] |
| Journal of Ginseng Research | Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut–liver axis of alcoholic liver disease [Pubmed:25378990] |
| BMC Immunology | The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases [Pubmed:25406630] |
| Gastroenterology Research and Practice | Procoagulatory State in Inflammatory Bowel Diseases Is Promoted by Impaired Intestinal Barrier Function [Pubmed:25767508] |
| Eur J Gastroenterol Hepatol | Efficacy of rifaximin on circulating endotoxins and cytokines in patients with nonalcoholic fatty liver disease [PubMed: 26043290] |
| Pathol Biol (Paris) | Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice [PubMed: 25834936] |
| Journal of Food Science | Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine [Pubmed:27061742] |
| Plos One | Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. [Pubmed:27223696] |
| Open Forum Infect Dis | The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients [Pubmed:26835476] |
| Animal Feed Science and Technology | Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindCavia (Guinea pig )t of dairy cows [science:S0377840115300493] |
| european journal of pharmacology | Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041] |
| european journal of pharmacology | S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948] |
| Psychopharmacology (Berl) | Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. [pubmed:28303372] |
| ЭНИ Забайкальский медицинский вестник | НОСИТЕЛЬСТВО ПОЛИМОРФИЗМА Thr399Ile и Asp299Gly ГЕНА TLR4 И УРОВЕНЬ TLR4 В ОПУХОЛЕВОЙ ТКАНИ У БОЛЬНЫХ ПРОЛИФЕРАТИВНЫМИ ЗАБОЛЕВАНИЯМИ МОЛОЧНОЙ ЖЕЛЕЗЫ [8.pdf] |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
| Journal For Neurovirology | Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. [pubmed:27882497] |
| Acta Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints [pubmed:28093922] |
| Environmental Toxicology and Pharmacology | Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study [pubmed:28192751] |
| Inflammation | Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats [pubmed:28690610] |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts [DOI: 10.1016/j.cimid.2017.06.002] |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts [10.1016/j.cimid.2017.06.002] |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
| Frontiers in Immunology | The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease Biomarker [Pubmed:29593720] |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia [Pubmed:30071285] |
| Neurotoxicity Research | Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells [Pubmed:29869772] |
| European Journal of Pharmacology | Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1 [Pubmed:29920283] |
| Environmental Toxicology and Pharmacology | Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity [Pubmed:29625388] |
| Physiology & Behavior | Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … [Pubmed:29410018] |
| International Journal of Gynecology & Obstetrics | Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia [Pubmed:29569400] |
| Journal of Maternal-Fetal & Neonatal Medicine | Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory state [Pubmed:29534643] |
| ТLR4-РІВНІ У ХВОРИХ НА ПОДАГРУ: ЗВ'ЯЗОК З АКТИВНІСТЮ ЗАХВОРЮВАННЯ [:] | |
| Journal of Medical Virology | Combined effect of anti‐high‐mobility group box‐1 monoclonal antibody and peramivir against influenza A virus‐induced pneumonia in mice [Pubmed: 30281823] |
| Metabolic Brain Disease | Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms [Pubmed: 30456649] |
| Experimental and Therapeutic Medicine | The HMGB1‑RAGE/TLR‑TNF‑α signaling pathway may contribute to kidney injury induced by hypoxia [] |
| Immunopharmacology and immunotoxicology | Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis [Pubmed: 30488751] |
| International Immunopharmacology | S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation [Pubmed: 30665040] |
| Biomedicine & Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 [] |
| International Journal of Obesity | Circulating microbiota-derived metabolites: a “liquid biopsy? [Pubmed: 31388096] |
| Scientific Reports | Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndrome [Pubmed: 31363123] |
| Life Sciences | Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis [Pubmed: 31465731] |
| Respiratory physiology & neurobiology | Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis [Pubmed: 31600583] |
| Thrombosis and haemostasis | Particulate Shiga Toxin 2 in Blood is Associated to the Development of Hemolytic Uremic Syndrome in Children [Pubmed: 31858520] |
| J Ethnopharmacol | Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea [Pubmed: 33217518] |
| International Journal of Molecular Sciences | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis [Pubmed: 32545403] |
| NEUROPSYCHOBIOLOGY | Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms [Pubmed: 32526752] |
| journal of biochemical and molecular toxicology | S‐allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d‐galactosamine in mouse: Underlying mechanisms [Pubmed: 32453893] |
| microbial pathogenesis | Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 mice [Pubmed: 32389705] |
| Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … [] |
| Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial … [] | |
| The role of Toll-like receptors-4 in the pathogenesis of development of anemia of inflammation in young children [] | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces [34091792] |
| Eksp Klin Gastroenterol | Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis [17539352] |
| Iranian Journal of Medical Sciences | Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice [] |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis [34244993] |
| Nutrients | Expression of Jejunal Taste Receptors in Women with Morbid Obesity [34371946] |
| Biomed Pharmacother | Nifuroxazide-loaded cubosomes exhibit an advancement in pulmonary delivery and attenuate bleomycin-induced lung fibrosis by regulating the STAT3 and … [Pubmed:35220029] |
| Biomed Pharmacother | Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an … [Pubmed:35202914] |
| Frontiers in Pharmacology | Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis. Front … [Pubmed:35754464] |
| Processes | Lipocalin, Resistin and Gut Microbiota-Derived Propionate Could Be Used to Predict Metabolic Bariatric Surgery Selected Outcomes [] |
| Biomolecules | Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study [] |
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