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聚集蛋白聚糖(AGC)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Aggrecan (AGC)

ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

  • 聚集蛋白聚糖(AGC)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 聚集蛋白聚糖(AGC)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对聚集蛋白聚糖(AGC)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPB908Hu02 聚集蛋白聚糖(AGC)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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PAB908Hu03 聚集蛋白聚糖(AGC)多克隆抗体 WB; IHC; ICC; IP.
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KSB908Hu01 聚集蛋白聚糖(AGC)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source]
Age( dordr). In vitro tenocyte metabolism in aging and oestrogen deficiency [Springer: Source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Stem Cell Research & Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. [Pubmed: 24739658]
Materials Science and Engineering: C Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition [ScienceDirect: S0928493113006656]
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
Macromolecular Bioscience Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration [PubMed: 25787871]
Osteoarthritis and Cartilage Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs [Pubmed:26802547]
Cell Proliferation Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes [Pubmed:27090063]
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731]
Journal of Clinical Medicine Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors [Pubmed: 32392807]
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs []
researchsquare Aggrecan-a new biomarker for acute type A aortic dissection [33990642]
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